502601

antibody from BioLegend

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Other assay (Recommended by provider)

Antibody Data

Product number

502601

Provider

BioLegend

Proper citation

BioLegend Cat#502601, RRID:AB_315240

Product name

Purified anti-human MCP-1

Antibody type

Monoclonal

Antigen

Recombinant human MCP-1

Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

ELISA

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Immunoprecipitation/Western blot analysis of capturing ability of MCP-1 antibody for 200ng/ml MCP-1 recombinant protein. Lane 1 was immunoprecipitated with Armenian hamster IgG isotype control antibody and lane 2 was immunoprecipitated with MCP-1 antibody (2H5) and lane 3 was immunoprecipitated with MCP-1(5D3-F7) . Western blot analysis was performed using mouse anti-MCP-1 antibody (5D3-F7).

Conjugate

Unconjugated