502606

antibody from BioLegend

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Recommended by provider)

ELISA (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Recommended by provider)

Blocking/Neutralizing (Recommended by provider)

Other assay (Recommended by provider)

Antibody Data

Product number

502606

Provider

BioLegend

Product name

LEAF™ Purified anti-human MCP-1 [5D3-F7]

Antibody type

Monoclonal

Antigen

Recombinant human MCP-1

Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 5D3-F7 antibody reacts with human monocyte chemoattractant protein-1 (MCP-1).

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 µg

Concentration

1 mg/ml

Storage

The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

ELISA

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Immunoprecipitation/Western blot analysis of capturing ability of MCP-1 antibody for 200ng/ml MCP-1 recombinant protein. Lane 1 was immunoprecipitated with Armenian hamster IgG isotype control antibody and lane 2 was immunoprecipitated with MCP-1 antibody (2H5) and lane 3 was immunoprecipitated with MCP-1(5D3-F7) . Western blot analysis was performed using mouse anti-MCP-1 antibody (5D3-F7).

Conjugate

Unconjugated