M2MCP1I

antibody from Invitrogen Antibodies

Targeting: CCL2 GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

M2MCP1I

Provider

Invitrogen Antibodies

Product name

MCP-1 Monoclonal Antibody (E10052)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

M2MCP1I targets MCP-1 in ELISA applications and shows reactivity with Human samples.

Antibody clone number

E10052

Concentration

1.00 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Pancreas (Lane 1), Mouse Spleen (Lane 2), Mouse Lung (Lane 3) and Mouse Cerebellum (Lane 4). The blots were probed with Anti-MCP-1 Mouse monoclonal Antibody (Product # M2MCP1I, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). A ~ 25 kDa band corresponding to MCP-1 was observed across cell lines. A shift in molecular weight is due to the glycosylation of MCP-1 protein. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).