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LearnAF-279-NA
antibody from R&D Systems
Targeting: CCL2
GDCF-2, HC11, MCAF, MCP-1, MCP1, MGC9434, SCYA2, SMC-CF
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-279-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL2/JE/MCP-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant canine MCP-1 is observed and less than 5% cross-reactivity with recombinant mouse MCP-1, recombinant rat MCP-1, recombinant human (rh) Eotaxin, rhMCP-2 and rhMCP-4 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P13500- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Autophagy facilitates TLR4- and TLR3-triggered migration and invasion of lung cancer cells through the promotion of TRAF6 ubiquitination.
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Interleukin-18 predicts atherosclerosis progression in SIV-infected and uninfected rhesus monkeys (Macaca mulatta) on a high-fat/high-cholesterol diet.
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
Alterations in expression of monocyte chemotactic protein-1 in the simian immunodeficiency virus model of disseminated Mycobacterium avium complex.
Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
Zhan Z, Xie X, Cao H, Zhou X, Zhang XD, Fan H, Liu Z
Autophagy 2014 Feb;10(2):257-68
Autophagy 2014 Feb;10(2):257-68
Fractalkine is a novel human adipochemokine associated with type 2 diabetes.
Shah R, Hinkle CC, Ferguson JF, Mehta NN, Li M, Qu L, Lu Y, Putt ME, Ahima RS, Reilly MP
Diabetes 2011 May;60(5):1512-8
Diabetes 2011 May;60(5):1512-8
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Ouwehand K, Oosterhoff D, Breetveld M, Scheper RJ, de Gruijl TD, Gibbs S
The Journal of investigative dermatology 2011 Feb;131(2):418-25
The Journal of investigative dermatology 2011 Feb;131(2):418-25
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
International journal of cancer 2009 Sep 15;125(6):1276-84
International journal of cancer 2009 Sep 15;125(6):1276-84
Interleukin-18 predicts atherosclerosis progression in SIV-infected and uninfected rhesus monkeys (Macaca mulatta) on a high-fat/high-cholesterol diet.
Yearley JH, Xia D, Pearson CB, Carville A, Shannon RP, Mansfield KG
Laboratory investigation; a journal of technical methods and pathology 2009 Jun;89(6):657-67
Laboratory investigation; a journal of technical methods and pathology 2009 Jun;89(6):657-67
A potential role of thymic stromal lymphopoietin in the recruitment of macrophages to mouse intervertebral disc cells via monocyte chemotactic protein 1 induction: implications for herniated discs.
Ohba T, Haro H, Ando T, Koyama K, Hatsushika K, Suenaga F, Ohnuma Y, Nakamura Y, Katoh R, Ogawa H, Hamada Y, Nakao A
Arthritis and rheumatism 2008 Nov;58(11):3510-9
Arthritis and rheumatism 2008 Nov;58(11):3510-9
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3277-90
The Journal of neuroscience : the official journal of the Society for Neuroscience 2008 Mar 26;28(13):3277-90
Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
The Journal of experimental medicine 2004 May 3;199(9):1213-21
The Journal of experimental medicine 2004 May 3;199(9):1213-21
Alterations in expression of monocyte chemotactic protein-1 in the simian immunodeficiency virus model of disseminated Mycobacterium avium complex.
Hendricks EE, Lin KC, Boisvert K, Pauley D, Mansfield KG
The Journal of infectious diseases 2004 May 1;189(9):1714-20
The Journal of infectious diseases 2004 May 1;189(9):1714-20
Inhibition of neointimal hyperplasia after balloon injury by cis-element 'decoy' of early growth response gene-1 in hypercholesterolemic rabbits.
Ohtani K, Egashira K, Usui M, Ishibashi M, Hiasa KI, Zhao Q, Aoki M, Kaneda Y, Morishita R, Takeshita A
Gene therapy 2004 Jan;11(2):126-32
Gene therapy 2004 Jan;11(2):126-32
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human CCL2/JE/MCP-1 by Western Blot. Western blot shows 25 ng of Recombinant Human CCL2/JE/MCP-1 (Catalog # 279-MC), Recombinant Mouse CCL2/JE/MCP-1 (Catalog # 479-JE), Recombinant Rat CCL2/JE/MCP-1 (Catalog # 3144-JE), Recombinant Human CCL7/MCP-3/MARC (Catalog # 282-P3), Recombinant Human CCL11/Eotaxin (Catalog # 320-EO), Recombinant Human CCL8/MCP-2 (Catalog # 281-CP), and Recombinant Human CCL13/MCP-4 (Catalog # 327-P4). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for CCL2/JE/MCP-1 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CCL2/JE/MCP-1 in Human Crohn's Disease Intestine. CCL2/JE/MCP-1 was detected in immersion fixed paraffin-embedded sections of human Crohn's disease intestine using Goat Anti-Human CCL2/JE/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL2/MCP-1 and Neutral-ization by Human CCL2/ MCP-1 Antibody. Recombinant Human CCL2/ MCP-1 (Catalog # 279-MC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL2/MCP-1 (150 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL2/MCP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-279-NA). The ND50 is typically 1.5-4.5 µg/mL.
