HPA019697

PSIP1 antibody from Atlas Antibodies

DFS70, LEDGF, p52, p75, PSIP2

Immunocytochemistry

Enhanced validation

Immunohistochemistry

Enhanced validation

Provider product page for HPA019697

Antibody Data

Product number

HPA019697

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA019697, RRID:AB_1852779

Product name

Anti-PSIP1

Provider product page

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KQVETEEAGVVTTATASVNLKVSPKRGRPAATEVK
IPKPRGRPKMVKQPCPSESDIITEEDKSKKKGQEE
KQPKKQPKKDEEGQ


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Sample type

HUMAN

Enhanced validation

Submitted by

55af80e3e0991

Enhanced method

Genetic validation

Main image

Experimental details

Confocal images of immunofluorescently stained human U-2 OS cells.The protein PSIP1 is shown in green and the microtubules in red. The image to the left show cells transfected with control siRNA and the image to the right show cells where PSIP1 has been downregulated with specific siRNA.

Sample type

U-2 OS cells

Primary Ab dilution

1:172

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:800

Knockdown/Genetic Approaches Application

Immunocytochemistry

KD Reagent Type

siRNA

Downregulation

>50%

KD Reagent Prod no

s22036

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no 2

s22037

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s22035

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R08619

Appendix

55b877be482ff.pdf

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Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows low expression as expected.

Sample type

HUMAN

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human cerebral cortex and liver tissues using Anti-PSIP1 antibody. Corresponding PSIP1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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