PA5-54060

antibody from Invitrogen Antibodies

Targeting: PSIP1 DFS70, LEDGF, p52, p75, PSIP2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54060

Provider

Invitrogen Antibodies

Product name

PSIP1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KQVETEEAGV VTTATASVNL KVSPKRGRPA ATEVKIPKPR GRPKMVKQPC PSESDIITEE DKSKKKGQEE KQPKKQPKKD EEGQ

Concentration

0.4 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of PSIP1 in human cell line U-2 OS shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a PSIP1 Polyclonal Antibody (Product # PA5-54060).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PSIP1 was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PSIP1 Rabbit Polyclonal Antibody (Product # PA5-54060) at 4 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PSIP1 in human cerebral cortex tissue shows strong nuclear as well as cytoplasmic positivity in neuronal and glial cells. Samples were probed using a PSIP1 Polyclonal Antibody (Product # PA5-54060).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PSIP1 in human cerebral cortex and liver tissues using PSIP1 Polyclonal Antibody (Product # PA5-54060). Corresponding PSIP1 RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of PSIP1 in human liver using PSIP1 Polyclonal Antibody (Product # PA5-54060) shows low expression as expected.