A300-847A

antibody from Invitrogen Antibodies

Targeting: PSIP1 DFS70, LEDGF, p52, p75, PSIP2

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-847A

Provider

Invitrogen Antibodies

Product name

LEDGF/p75 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (A300-847A lot 3) used at 1:5000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human LEDGF/p75 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF/p75 antibody A300-847A (lot A300-847A-3) used for IP at 3 µg per reaction. LEDGF/p75 was also immunoprecipitated by rabbit anti-LEDGF/p75 antibody A300-848A. For blotting immunoprecipitated LEDGF-p75, A300-847A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.