Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunoprecipitation [2]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB110-40560 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-40560, RRID:AB_669585
- Product name
- Rabbit Polyclonal LEDGF Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Expression of the stress response oncoprotein LEDGF/p75 in human cancer: a study of 21 tumor types.
The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering.
Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ.
Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity.
LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration.
Basu A, Rojas H, Banerjee H, Cabrera IB, Perez KY, De León M, Casiano CA
PloS one 2012;7(1):e30132
PloS one 2012;7(1):e30132
The transcriptional co-activator LEDGF/p75 displays a dynamic scan-and-lock mechanism for chromatin tethering.
Hendrix J, Gijsbers R, De Rijck J, Voet A, Hotta J, McNeely M, Hofkens J, Debyser Z, Engelborghs Y
Nucleic acids research 2011 Mar;39(4):1310-25
Nucleic acids research 2011 Mar;39(4):1310-25
Lens epithelium-derived growth factor/p75 interacts with the transposase-derived DDE domain of PogZ.
Bartholomeeusen K, Christ F, Hendrix J, Rain JC, Emiliani S, Benarous R, Debyser Z, Gijsbers R, De Rijck J
The Journal of biological chemistry 2009 Apr 24;284(17):11467-77
The Journal of biological chemistry 2009 Apr 24;284(17):11467-77
Identification and characterization of PWWP domain residues critical for LEDGF/p75 chromatin binding and human immunodeficiency virus type 1 infectivity.
Shun MC, Botbol Y, Li X, Di Nunzio F, Daigle JE, Yan N, Lieberman J, Lavigne M, Engelman A
Journal of virology 2008 Dec;82(23):11555-67
Journal of virology 2008 Dec;82(23):11555-67
LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration.
Shun MC, Raghavendra NK, Vandegraaff N, Daigle JE, Hughes S, Kellam P, Cherepanov P, Engelman A
Genes & development 2007 Jul 15;21(14):1767-78
Genes & development 2007 Jul 15;21(14):1767-78
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of Human LEDGF/p75 on HeLa whole cell lysate using NB110-40560 used for IP at 3 mcg/mg lysate. LEDGF/p75 was also IPed by another rabbit anti-LEDGF/p75 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: LEDGF Antibody [NB110-40560] - Detection of human LEDGF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-LEDGF antibody NB110-40560 used for IP at 3 ug per reaction. LEDGF was also immunoprecipitated by rabbit anti-LEDGF antibody NB110-40561. For blotting immunoprecipitated LEDGF, NB110-40560 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: LEDGF Antibody [NB110-40560] - Sample: FFPE sections of human lung carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti-fs18 LEDGF/p75 fs20 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: LEDGF Antibody [NB110-40560] - Detection of human LEDGF/p75 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-LEDGF/p75 antibody (NB110-40560). Secondary: HRP-conjugated goat anti-rabbit IgG.
