Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- AF3468 - Provider product page
- Provider
- R&D Systems
- Product name
- Human LEDGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LEDGF in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O75475
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LEDGF by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and human CD4+ T cells. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human LEDGF by Simple WesternTM. Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for LEDGF p75 at approximately 99 kDa (as indicated) using 5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Western Blot Shows Human LEDGF Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and LEDGF knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for LEDGF p75 at approximately 75 kDa and LEDGF p52 at approximately 49 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LEDGF in HeLa Human Cell Line. LEDGF was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human LEDGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3468) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.