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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB2504 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2504, RRID:AB_1572587
- Product name
- ACSL4 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ACSL4.
- Antigen sequence
IHSMQSVEELGSNPENLGIPPSRPTPSDMAI- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An evaluation of fatty acid-CoA ligase 4 in breast cancer.
ACSL3 and GSK-3β are essential for lipid upregulation induced by endoplasmic reticulum stress in liver cells.
Tsai CH, Sheen-Chen SM, Huang CY, Chen YJ, Chi SY, Ko SF, Lee YW, Liang JL, Hsu HW, Huang EY, Chen HC, Huang CC, Rau KM, Tang RP
Anticancer research 2014 Mar;34(3):1239-43
Anticancer research 2014 Mar;34(3):1239-43
ACSL3 and GSK-3β are essential for lipid upregulation induced by endoplasmic reticulum stress in liver cells.
Chang YS, Tsai CT, Huangfu CA, Huang WY, Lei HY, Lin CF, Su IJ, Chang WT, Wu PH, Chen YT, Hung JH, Young KC, Lai MD
Journal of cellular biochemistry 2011 Mar;112(3):881-93
Journal of cellular biochemistry 2011 Mar;112(3):881-93
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The ACSL4 polyclonal antibody (Cat # PAB2504) is used in Western blot to detect ACSL4 in RKO cell lysate (human colon cancer) treated with 10% FCS for 24 hrs. Data is kindly provided by Drs. M.G. Sciulli and P. Patrignani from the University of Chieti (Italy).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of HepG2 whole cell lysate with ACSL4 polyclonal antibody (Cat # PAB2504) followed by HRP conjugated goat anti-rabbit IgG (H+L) as second antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with the ACSL4 polyclonal antibody (Cat # PAB2504) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
