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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- PA5-93240 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ACSL4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MAKRIKAKPT SDKPGSPYRS VTHFDSLAVI DIPGADTLDK LFDHAVSKFG KKDSLGTREI LSEENEMQPN GKVFKKLILG NYKWMNYLEV NRRVNNFGSG LTALGLKPKN TIAIFCETRA EWMIAAQTCF KYNFPLVTLY ATLGKEAVVH GLNESEASYL ITSVELLESK LKTALLDISC VKHIIYVDNK AINKAEYPEG FEIHSMQSVE ELGSNPENLG IPPSRPTPSD MAIVMYTSGS TGRPKGVMMH HSNLIAGMTG QCERIPGLGP KDTYIGYLPL; Positive Samples: HeLa, HT-1080, 22Rv1, Mouse liver; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Mitochondrion outer membrane, Peroxisome membrane, Single-pass type III membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.26 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of ACSL4 in NIH/3T3 cells. Samples were incubated with ACSL4 Polyclonal antibody (Product # PA5-93240) using a dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ACSL4 in 300 μg extracts of HepG2 cells. Samples were precipitated with 3 μg ACSL4 Polyclonal antibody (Product # PA5-93240). Western blot was performed from the immunoprecipitate using ACSL4 Polyclonal antibody (Product # PA5-93240) at a dilution of 1:2,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ACSL4 in paraffin-embedded mouse kidney. Samples were incubated with ACSL4 Polyclonal antibody (Product # PA5-93240) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ACSL4 in paraffin-embedded rat brain. Samples were incubated with ACSL4 Polyclonal antibody (Product # PA5-93240) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
