12295-1-AP

antibody from Proteintech Group

Targeting: PHB2 Bap37, BCAP37, p22, REA

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

12295-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12295-1-AP, RRID:AB_2164779

Product name

Prohibitin 2 antibody

Antibody type

Polyclonal

Description

KD/KO validated Prohibitin 2 antibody (Cat. #12295-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Sensitive electrochemical immunosensor to detect prohibitin 2, a potential blood cancer biomarker.

Yun YR, Lee SY, Seo B, Kim H, Shin MG, Yang S
Talanta 2022 Feb 1;238(Pt 2):123053

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Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.

Chamberlain KA, Huang N, Xie Y, LiCausi F, Li S, Li Y, Sheng ZH
Neuron 2021 Nov 3;109(21):3456-3472.e8

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Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.

Wang W, Zhai D, Bai Y, Xue K, Deng L, Ma L, Du T, Ye Z, Qu D, Xiang A, Chen G, Zhao Y, Wang L, Lu Z
Cell death discovery 2021 Mar 23;7(1):58

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Nitazoxanide impairs mitophagy flux through ROS-mediated mitophagy initiation and lysosomal dysfunction in bladder cancer.

Sun H, Ou T, Hu J, Yang Z, Lei Q, Li Y, Wang G, Li Y, Wu K, Wang S, Wu S
Biochemical pharmacology 2021 Aug;190:114588

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BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.

Wu X, Zheng Y, Liu M, Li Y, Ma S, Tang W, Yan W, Cao M, Zheng W, Jiang L, Wu J, Han F, Qin Z, Fang L, Hu W, Chen Z, Zhang X
Autophagy 2021 Aug;17(8):1934-1946

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SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2.

von Wenserski L, Schultheiß C, Bolz S, Schliffke S, Simnica D, Willscher E, Gerull H, Wolters-Eisfeld G, Riecken K, Fehse B, Altfeld M, Nollau P, Binder M
Leukemia 2021 Apr;35(4):1073-1086

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ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.

Shu L, Hu C, Xu M, Yu J, He H, Lin J, Sha H, Lu B, Engelender S, Guan M, Song Z
The EMBO journal 2021 Apr 15;40(8):e106283

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PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Yan C, Gong L, Chen L, Xu M, Abou-Hamdan H, Tang M, Désaubry L, Song Z
Autophagy 2020 Mar;16(3):419-434

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Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA.

Hao Q, Wang P, Dutta P, Chung S, Li Q, Wang K, Li J, Cao W, Deng W, Geng Q, Schrode K, Shaheen M, Wu K, Zhu D, Chen QH, Chen G, Elshimali Y, Vadgama J, Wu Y
Cell death & disease 2020 Dec 11;11(12):1052

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Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.

Cho SG, Xiao X, Wang S, Gao H, Rafikov R, Black S, Huang S, Ding HF, Yoon Y, Kirken RA, Yin XM, Wang HG, Dong Z
Journal of the American Society of Nephrology : JASN 2019 Jul;30(7):1174-1191

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Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

Han Y, Wu P, Wang Z, Zhang Z, Sun S, Liu J, Gong S, Gao P, Iwakuma T, Molina-Vila MA, Chen BP, Zhang Y, Ji T, Mo Q, Chen P, Hu J, Wang S, Zhou J, Lu H, Gao Q
EBioMedicine 2019 Feb;40:92-105

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NAD⁺ repletion inhibits the endothelial-to-mesenchymal transition induced by TGF-β in endothelial cells through improving mitochondrial unfolded protein response.

Zhang M, Weng H, Zheng J
The international journal of biochemistry & cell biology 2019 Dec;117:105635

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Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.

Yahiro K, Ogura K, Terasaki Y, Satoh M, Miyagi S, Terasaki M, Yamasaki E, Moss J
Cellular microbiology 2019 Aug;21(8):e13033

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Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005

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PHB2 interacts with LC3 and SQSTM1 is required for bile acids-induced mitophagy in cholestatic liver.

Xiao Y, Zhou Y, Lu Y, Zhou K, Cai W
Cell death & disease 2018 Feb 7;9(2):160

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Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92

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Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.

Otera H, Miyata N, Kuge O, Mihara K
The Journal of cell biology 2016 Feb 29;212(5):531-44

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Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

Poitelon Y, Bogni S, Matafora V, Della-Flora Nunes G, Hurley E, Ghidinelli M, Katzenellenbogen BS, Taveggia C, Silvestri N, Bachi A, Sannino A, Wrabetz L, Feltri ML
Nature communications 2015 Sep 18;6:8303

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Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian (HL)-type cytoplasmic male sterility rice.

Liu G, Tian H, Huang YQ, Hu J, Ji YX, Li SQ, Feng YQ, Guo L, Zhu YG
The Journal of biological chemistry 2012 Nov 16;287(47):40051-60

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Proteomic analysis identifies in vivo candidate matrix metalloproteinase-9 substrates in the left ventricle post-myocardial infarction.

Zamilpa R, Lopez EF, Chiao YA, Dai Q, Escobar GP, Hakala K, Weintraub ST, Lindsey ML
Proteomics 2010 Jun;10(11):2214-23

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12295-1-AP(PHB2 antibody) at dilution of 1:1000

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PHB2 antibody 12295-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Sample type

cell line

Immunoprecipitation

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

IP Result of anti-PHB2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 12295-1-AP(PHB2 antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 12295-1-AP(PHB2 antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue