12295-1-AP

antibody from Proteintech Group

Targeting: PHB2 Bap37, BCAP37, p22, REA

Provider product page for 12295-1-AP

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ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [64]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunoprecipitation [1]
Immunohistochemistry [2]

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Product number: 12295-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#12295-1-AP, RRID:AB_2164779
Product name: Prohibitin 2 antibody
Antibody type: Polyclonal
Description: KD/KO validated Prohibitin 2 antibody (Cat. #12295-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PHB2 protein structure - 12295-1-AP shown in red.

Submitted references Complement protein C1q induces endothelial apoptosis through C1qBP regulation of mitochondrial function in lung endothelial cells in systemic sclerosis.

Yi Z, Liu K, Shabin M
Molecular medicine (Cambridge, Mass.) 2026 Mar 5;32(1)

Inhibition of PHB1/PHB2 suppresses atherosclerotic plaque formation by interrupting PI3K/AKT/mTOR signaling.

Li M, Hu X, Hu X, Gao F, Cui Y, Wei X, Qin Y, An X, Zhao Y, Gao Y
PloS one 2025;20(4):e0320509

The ubiquitin-binding protein ANKRD13A mediates VCP-dependent mitochondrial outer membrane rupture during PINK1/Parkin-mediated mitophagy.

Chu WH, Lin YS, Guo J, Jane WN, Wang WJ, Lin YY, Liu PH, Huang PY, Chiang WC
The Journal of biological chemistry 2025 Nov;301(11):110739

The ER thioredoxin-related transmembrane protein TMX2 controls redox-mediated tethering of ER-mitochondria contacts.

Chen J, Yap MC, Bassot A, Pascual DM, Makio T, Zimmermann J, Mast H, Bhat R, Fleury SG, Fan Y, Buzatto AZ, Moore J, Ballanyi K, Li L, Overduin M, Lemieux MJ, Lemieux H, Mancini GMS, Morgan B, Marcogliese PC, Simmen T
Cell reports 2025 Nov 25;44(11):116486

Nrf2 alleviates excessive deposition of extracellular matrix in mammary fibrosis through TGF/Smad and ROS signals.

Kan X, Xu P, Yu M, Guo W, Cao Y, Hu G, Liu J, Fu S, Bi J
Scientific reports 2025 Nov 10;15(1):39252

Krüppel like factor 7 regulates mitochondrial dynamics balance in myocardial infarction.

Wang C, Zhu F, Zhou L, Zhang S, Wang R, Tian H, Zhang B, Wu J, Xu X, Jiang R, Hou X, Liu J, Tian W
Communications biology 2025 May 9;8(1):722

A large-scale sORF screen identifies putative microproteins involved in cancer cell fitness.

Schlesinger D, Dirks C, Navarro C, Lafranchi L, Spinner A, Raja GL, Mun-Sum Tong G, Eirich J, Martinez TF, Elsässer SJ
iScience 2025 Mar 21;28(3):111884

Salmonella Typhimurium persistently infects host via its effector SseJ-induced PHB2-mediated mitophagy.

Sun D, Gou H, Zhang Y, Li J, Dai C, Shen H, Chen K, Wang Y, Pan P, Zhu T, Xu C, Shan T, Liao M, Zhang J
Autophagy 2025 Jun;21(6):1228-1244

Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17.

Jian F, Wang S, Tian W, Chen Y, Wang S, Li Y, Ma C, Rong Y
Nature communications 2025 Jan 9;16(1):543

HIV-1 gp120 Interactions with Nicotine Modulate Mitochondrial Network Properties and Amyloid Release in Microglia.

Graur A, Erickson N, Sinclair P, Nusir A, Kabbani N
Neurochemical research 2025 Feb 24;50(2):103

Snhg18 regulates Yap subcellular localization to maintain bone homeostasis.

Huang J, Weng Y, Feng Y, Wu D, Chen Y, Li Z, Jiang X, Wang H, Wang Z
Nature communications 2025 Aug 14;16(1):7543

The tumor suppressor Parkin exerts anticancer effects through regulating mitochondrial GAPDH activity.

Sun X, Ye G, Li J, Yuan L, Bai G, Xu YJ, Zhang J
Oncogene 2024 Oct;43(44):3215-3226

N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression.

Sun L, Li Z, Shu P, Lu M
Cancer science 2024 Oct;115(10):3320-3332

High glucose- or AGE-induced oxidative stress inhibits hippocampal neuronal mitophagy through the Keap1-Nrf2-PHB2 pathway in diabetic encephalopathy.

Xu S, Gao Z, Jiang L, Li J, Qin Y, Zhang D, Tian P, Wang W, Zhang N, Zhang R, Xu S
Scientific reports 2024 Oct 14;14(1):24044

Identification and characterization of a novel small viral peptide (VSP59) encoded by Bombyx mori cypovirus (BmCPV) that negatively regulates viral replication.

Cao M, Qiu Q, Zhang X, Zhang W, Shen Z, Ma C, Zhu M, Pan J, Tong X, Cao G, Gong C, Hu X
Microbiology spectrum 2024 Nov 5;12(11):e0082624

Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria.

Poerschke S, Oeljeklaus S, Cruz-Zaragoza LD, Schenzielorz A, Dahal D, Hillen HS, Das H, Kremer LS, Valpadashi A, Breuer M, Sattmann J, Richter-Dennerlein R, Warscheid B, Dennerlein S, Rehling P
Molecular cell 2024 Jan 18;84(2):345-358.e5

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer.

Xu L, Xiang W, Yang J, Gao J, Wang X, Meng L, Ye K, Zhao XH, Zhang XD, Jin L, Ye Y
Journal of experimental & clinical cancer research : CR 2024 Jan 11;43(1):17

The STX17-SNAP47-VAMP7/VAMP8 complex is the default SNARE complex mediating autophagosome-lysosome fusion.

Jian F, Wang S, Tian R, Wang Y, Li C, Li Y, Wang S, Fang C, Ma C, Rong Y
Cell research 2024 Feb;34(2):151-168

CRISPR-editing of the virus vector Aedes albopictus cell line C6/36, illustrated by prohibitin 2 gene knockout.

Chan SW
MethodsX 2024 Dec;13:102817

Exosome-delivered NR2F1-AS1 and NR2F1 drive phenotypic transition from dormancy to proliferation in treatment-resistant prostate cancer via stabilizing hormonal receptors.

Chen W, Mao Y, Zhan Y, Li W, Wu J, Mao X, Xu B, Shu F
Journal of nanobiotechnology 2024 Dec 18;22(1):761

GOLPH3 inhibits erastin-induced ferroptosis in colorectal cancer cells.

Chen L, Wang C, Chen X, Wu Y, Chen M, Deng X, Qiu C
Cell biology international 2024 Aug;48(8):1198-1211

A novel mechanism of PHB2-mediated mitophagy participating in the development of Parkinson's disease.

Zhang Y, Yin S, Song R, Lai X, Shen M, Wu J, Yan J
Neural regeneration research 2024 Aug 1;19(8):1828-1834

SS-31 inhibits mtDNA-cGAS-STING signaling to improve POCD by activating mitophagy in aged mice.

Ji Y, Ma Y, Ma Y, Wang Y, Zhao X, Jin D, Xu L, Ge S
Inflammation research : official journal of the European Histamine Research Society ... [et al.] 2024 Apr;73(4):641-654

Chaihu Shugan Powder inhibits interstitial cells of cajal mitophagy through USP30 in the treatment of functional dyspepsia.

Wang X, Liu X, Wang Y, Yang K, Yeertai Y, Jia Q, Li L, Jiang K, Du G, Ling J
Journal of ethnopharmacology 2024 Apr 6;323:117695

13-oxyingenol dodecanoate derivatives induce mitophagy and ferroptosis through targeting TMBIM6 as potential anti-NSCLC agents.

Wang Y, Gu L, Li J, Wang R, Zhuang Y, Li X, Wang X, Zhang J, Liu Q, Wang J, Song SJ
European journal of medicinal chemistry 2024 Apr 15;270:116312

PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function.

Yang M, Abudureyimu M, Wang X, Zhou Y, Zhang Y, Ren J
Redox biology 2023 Sep;65:102812

Decreasing expression of Prohibitin-2 lowers the oncogenicity of renal cell carcinoma cells by suppressing eIF4E-mediated oncogene translation via MNK inhibition.

Yang J, Li G, Huang Y, Liu Y
Toxicology and applied pharmacology 2023 May 1;466:116458

PHB2 promotes colorectal cancer cell proliferation and tumorigenesis through NDUFS1-mediated oxidative phosphorylation.

Ren L, Meng L, Gao J, Lu M, Guo C, Li Y, Rong Z, Ye Y
Cell death & disease 2023 Jan 20;14(1):44

Apolipoproteins L1 and L3 control mitochondrial membrane dynamics.

Lecordier L, Heo P, Graversen JH, Hennig D, Skytthe MK, Cornet d'Elzius A, Pincet F, Pérez-Morga D, Pays E
Cell reports 2023 Dec 26;42(12):113528

Flt3 Activation Mitigates Mitochondrial Fragmentation and Heart Dysfunction through Rebalanced L-OPA1 Processing by Hindering the Interaction between Acetylated p53 and PHB2 in Cardiac Remodeling.

Zhang K, Zheng Y, Bao G, Ma W, Han B, Shi H, Zhao Z
Antioxidants (Basel, Switzerland) 2023 Aug 22;12(9)

Profiling of purified autophagic vesicle degradome in the maturing and aging brain.

Kallergi E, Siva Sankar D, Matera A, Kolaxi A, Paolicelli RC, Dengjel J, Nikoletopoulou V
Neuron 2023 Aug 2;111(15):2329-2347.e7

Artemisinin Alleviates Cerebral Ischemia/Reperfusion-Induced Oxidative Damage via Regulating PHB2-Mediated Autophagy in the Human Neuroblastoma SH-SY5Y Cell Line.

Jiang M, Lai X, Zhang Y, Shen M, Ma H, Liu A, Wu J, Yan J
Oxidative medicine and cellular longevity 2022;2022:6568748

UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy.

Zheng J, Cao Y, Yang J, Jiang H
EMBO reports 2022 Oct 6;23(10):e54859

Loss of Sam50 in hepatocytes induces cardiolipin-dependent mitochondrial membrane remodeling to trigger mtDNA release and liver injury.

Chen L, Dong J, Liao S, Wang S, Wu Z, Zuo M, Liu B, Yan C, Chen Y, He H, Meng Q, Song Z
Hepatology (Baltimore, Md.) 2022 Nov;76(5):1389-1408

The Clostridium Metabolite P-Cresol Sulfate Relieves Inflammation of Primary Biliary Cholangitis by Regulating Kupffer Cells.

Fu HY, Xu JM, Ai X, Dang FT, Tan X, Yu HY, Feng J, Yang WX, Ma HT, Tu RF, Gupta AK, Manandhar LK, Bao WM, Tang YM
Cells 2022 Nov 26;11(23)

Identification of PHB2 as a Potential Biomarker of Luminal A Breast Cancer Cells Using a Cell-Specific Aptamer.

Liu M, Wang Z, Li S, Deng Y, He N
ACS applied materials & interfaces 2022 Nov 23;14(46):51593-51601

Mechanical detection of interactions between proteins related to intermediate filament and transcriptional regulation in living cells.

Yamagishi A, Mizusawa M, Uchida K, Iijima M, Kuroda S, Fukazawa K, Ishihara K, Nakamura C
Biosensors & bioelectronics 2022 Nov 15;216:114603

Effects of Wenyang Zhenshuai Granules on the Expression of Key Mitochondrial Autophagy Proteins in the Doxorubicin-Induced Model of H9c2 Cardiomyocyte Injury.

Peng LQ, Wu XX, Chen G, Cai HZ, Tang YP, Chen QY, Chen XY
Bulletin of experimental biology and medicine 2022 Jul;173(3):335-340

The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.

Cheng M, Yu H, Kong Q, Wang B, Shen L, Dong D, Sun L
International journal of molecular sciences 2022 Jan 25;23(3)

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Gourdomichali O, Zonke K, Kattan FG, Makridakis M, Kontostathi G, Vlahou A, Doxakis E
Biology 2022 Feb 11;11(2)

Sensitive electrochemical immunosensor to detect prohibitin 2, a potential blood cancer biomarker.

Yun YR, Lee SY, Seo B, Kim H, Shin MG, Yang S
Talanta 2022 Feb 1;238(Pt 2):123053

The interaction between E3 ubiquitin ligase Parkin and mitophagy receptor PHB2 links inner mitochondrial membrane ubiquitination to efficient mitophagy.

Sun S, Hou H, Ma G, Ma Q, Li N, Zhang L, Dong C, Cao M, Tam KY, Ying Z, Wang H
The Journal of biological chemistry 2022 Dec;298(12):102704

Development of artesunate intelligent prodrug liposomes based on mitochondrial targeting strategy.

Gu L, Zhang J, Liu D, Chen J, Liu S, Peng Q, Tian Y, Du M, Zhang J, Xiao W, Shen S, Wang J
Journal of nanobiotechnology 2022 Aug 13;20(1):376

Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.

Chamberlain KA, Huang N, Xie Y, LiCausi F, Li S, Li Y, Sheng ZH
Neuron 2021 Nov 3;109(21):3456-3472.e8

Discovery of bactericides as an acute mitochondrial membrane damage inducer.

Houston R, Sekine Y, Larsen MB, Murakami K, Mullett SJ, Wendell SG, Narendra DP, Chen BB, Sekine S
Molecular biology of the cell 2021 Nov 1;32(21):ar32

Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.

Wang W, Zhai D, Bai Y, Xue K, Deng L, Ma L, Du T, Ye Z, Qu D, Xiang A, Chen G, Zhao Y, Wang L, Lu Z
Cell death discovery 2021 Mar 23;7(1):58

Nitazoxanide impairs mitophagy flux through ROS-mediated mitophagy initiation and lysosomal dysfunction in bladder cancer.

Sun H, Ou T, Hu J, Yang Z, Lei Q, Li Y, Wang G, Li Y, Wu K, Wang S, Wu S
Biochemical pharmacology 2021 Aug;190:114588

BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.

Wu X, Zheng Y, Liu M, Li Y, Ma S, Tang W, Yan W, Cao M, Zheng W, Jiang L, Wu J, Han F, Qin Z, Fang L, Hu W, Chen Z, Zhang X
Autophagy 2021 Aug;17(8):1934-1946

SLAMF receptors negatively regulate B cell receptor signaling in chronic lymphocytic leukemia via recruitment of prohibitin-2.

von Wenserski L, Schultheiß C, Bolz S, Schliffke S, Simnica D, Willscher E, Gerull H, Wolters-Eisfeld G, Riecken K, Fehse B, Altfeld M, Nollau P, Binder M
Leukemia 2021 Apr;35(4):1073-1086

ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.

Shu L, Hu C, Xu M, Yu J, He H, Lin J, Sha H, Lu B, Engelender S, Guan M, Song Z
The EMBO journal 2021 Apr 15;40(8):e106283

PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.

Yan C, Gong L, Chen L, Xu M, Abou-Hamdan H, Tang M, Désaubry L, Song Z
Autophagy 2020 Mar;16(3):419-434

Sam50-Mic19-Mic60 axis determines mitochondrial cristae architecture by mediating mitochondrial outer and inner membrane contact.

Tang J, Zhang K, Dong J, Yan C, Hu C, Ji H, Chen L, Chen S, Zhao H, Song Z
Cell death and differentiation 2020 Jan;27(1):146-160

Comp34 displays potent preclinical antitumor efficacy in triple-negative breast cancer via inhibition of NUDT3-AS4, a novel oncogenic long noncoding RNA.

Hao Q, Wang P, Dutta P, Chung S, Li Q, Wang K, Li J, Cao W, Deng W, Geng Q, Schrode K, Shaheen M, Wu K, Zhu D, Chen QH, Chen G, Elshimali Y, Vadgama J, Wu Y
Cell death & disease 2020 Dec 11;11(12):1052

Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.

Cho SG, Xiao X, Wang S, Gao H, Rafikov R, Black S, Huang S, Ding HF, Yoon Y, Kirken RA, Yin XM, Wang HG, Dong Z
Journal of the American Society of Nephrology : JASN 2019 Jul;30(7):1174-1191

Ubiquinol-cytochrome C reductase core protein II promotes tumorigenesis by facilitating p53 degradation.

Han Y, Wu P, Wang Z, Zhang Z, Sun S, Liu J, Gong S, Gao P, Iwakuma T, Molina-Vila MA, Chen BP, Zhang Y, Ji T, Mo Q, Chen P, Hu J, Wang S, Zhou J, Lu H, Gao Q
EBioMedicine 2019 Feb;40:92-105

NAD(+) repletion inhibits the endothelial-to-mesenchymal transition induced by TGF-β in endothelial cells through improving mitochondrial unfolded protein response.

Zhang M, Weng H, Zheng J
The international journal of biochemistry & cell biology 2019 Dec;117:105635

Cholix toxin, an eukaryotic elongation factor 2 ADP-ribosyltransferase, interacts with Prohibitins and induces apoptosis with mitochondrial dysfunction in human hepatocytes.

Yahiro K, Ogura K, Terasaki Y, Satoh M, Miyagi S, Terasaki M, Yamasaki E, Moss J
Cellular microbiology 2019 Aug;21(8):e13033

Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.

Jian F, Chen D, Chen L, Yan C, Lu B, Zhu Y, Chen S, Shi A, Chan DC, Song Z
Cell reports 2018 Jun 5;23(10):2989-3005

PHB2 interacts with LC3 and SQSTM1 is required for bile acids-induced mitophagy in cholestatic liver.

Xiao Y, Zhou Y, Lu Y, Zhou K, Cai W
Cell death & disease 2018 Feb 7;9(2):160

Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.

Li H, Ruan Y, Zhang K, Jian F, Hu C, Miao L, Gong L, Sun L, Zhang X, Chen S, Chen H, Liu D, Song Z
Cell death and differentiation 2016 Mar;23(3):380-92

Drp1-dependent mitochondrial fission via MiD49/51 is essential for apoptotic cristae remodeling.

Otera H, Miyata N, Kuge O, Mihara K
The Journal of cell biology 2016 Feb 29;212(5):531-44

Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.

Poitelon Y, Bogni S, Matafora V, Della-Flora Nunes G, Hurley E, Ghidinelli M, Katzenellenbogen BS, Taveggia C, Silvestri N, Bachi A, Sannino A, Wrabetz L, Feltri ML
Nature communications 2015 Sep 18;6:8303

Alterations of mitochondrial protein assembly and jasmonic acid biosynthesis pathway in Honglian (HL)-type cytoplasmic male sterility rice.

Liu G, Tian H, Huang YQ, Hu J, Ji YX, Li SQ, Feng YQ, Guo L, Zhu YG
The Journal of biological chemistry 2012 Nov 16;287(47):40051-60

Proteomic analysis identifies in vivo candidate matrix metalloproteinase-9 substrates in the left ventricle post-myocardial infarction.

Zamilpa R, Lopez EF, Chiao YA, Dai Q, Escobar GP, Hakala K, Weintraub ST, Lindsey ML
Proteomics 2010 Jun;10(11):2214-23

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12295-1-AP(PHB2 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using PHB2 antibody 12295-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: IP Result of anti-PHB2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 12295-1-AP(PHB2 antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 12295-1-AP(PHB2 antibody) at dilution of 1:50 (under 40x lens)
Sample type: tissue