AP7270e

antibody from Abgent

Targeting: PHB2 Bap37, BCAP37, p22, REA

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7270e

Provider

Abgent

Proper citation

Abgent Cat#AP7270e, RRID:AB_1553827

Product name

PHB2 Antibody (Y248)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.44 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PHB2-Y248 (Cat. #AP7270e) in mouse kidney tissue lysates (35ug/lane). PHB2 (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of PHB2 (arrow) using PHB2 Antibody (Y248) (Cat. #AP7270e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

PHB2 Antibody (Y248)(Cat. #AP7270e) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PHB2 Antibody (Y248) for immunohistochemistry. Clinical relevance has not been evaluated.

Primary Ab dilution

1:50~100