Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2840 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2840, RRID:AB_1578684
- Product name
- PHB2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PHB2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references PHB2 protects sister-chromatid cohesion in mitosis.
Mitochondrial functions and estrogen receptor-dependent nuclear translocation of pleiotropic human prohibitin 2.
Takata H, Matsunaga S, Morimoto A, Ma N, Kurihara D, Ono-Maniwa R, Nakagawa M, Azuma T, Uchiyama S, Fukui K
Current biology : CB 2007 Aug 7;17(15):1356-61
Current biology : CB 2007 Aug 7;17(15):1356-61
Mitochondrial functions and estrogen receptor-dependent nuclear translocation of pleiotropic human prohibitin 2.
Kasashima K, Ohta E, Kagawa Y, Endo H
The Journal of biological chemistry 2006 Nov 24;281(47):36401-10
The Journal of biological chemistry 2006 Nov 24;281(47):36401-10
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PHB2 (arrow) using rabbit PHB2 polyclonal antibody (Cat # PAB2840). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 polyclonal antibody (Cat # PAB2840) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)