Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN359501 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Prohibitin 2 (PHB2) (N-Term) antibody
- Antibody type
- Polyclonal
- Antigen
- This antibody is generated from rabbits immunized with a KLH conjugated syntheticpeptide selected from the N-terminal region of human PHB2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
- Storage
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
Submitted references PHB2 protects sister-chromatid cohesion in mitosis.
Mitochondrial functions and estrogen receptor-dependent nuclear translocation of pleiotropic human prohibitin 2.
Takata H, Matsunaga S, Morimoto A, Ma N, Kurihara D, Ono-Maniwa R, Nakagawa M, Azuma T, Uchiyama S, Fukui K
Current biology : CB 2007 Aug 7;17(15):1356-61
Current biology : CB 2007 Aug 7;17(15):1356-61
Mitochondrial functions and estrogen receptor-dependent nuclear translocation of pleiotropic human prohibitin 2.
Kasashima K, Ohta E, Kagawa Y, Endo H
The Journal of biological chemistry 2006 Nov 24;281(47):36401-10
The Journal of biological chemistry 2006 Nov 24;281(47):36401-10
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of PHB2 (arrow) using rabbit polyclonal PHB2 Antibody (Human C-term) (AP13985PU-N). 293 cell lysates (2 μg/lane) either nontransfected (Lane 1) or transiently transfected with the PHB2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PHB2 Antibody (N-term) (AP13985PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.