- MA5-50793 - Invitrogen Antibodies YTHDF1 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of YTHDF1 in different various lysates. Lane 1: HepG2 cell lysate (10 µg/Lane); Lane 2: Hela cell lysate (10 µg/Lane); Lane 3: 293T cell lysate (10 µg/Lane); Lane 4: MCF-7 cell lysate (10 µg/Lane); Lane 5: NIH/3T3 cell lysate (10 µg/Lane); Lane 6: PC-12 cell lysate (10 µg/Lane); Lane 7: HCT116 cell lysate (10 µg/Lane); Lane 8: Mouse testis tissue lysate (20 µg/Lane); Lane 9: Rat testis tissue lysate (20 µg/Lane)Lane 10: Mouse brain tissue lysate (20 µg/Lane)Lane 11: Rat brain tissue lysate (20 µg/Lane). Predicted band size: 61 kDa. Observed band size: 69 kDa. Exposure time: 5 minutes; 10% SDS-PAGE gel. Primary antibody YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody with a dilution of 1:300,000 was used for 1 hour at room temperature. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of YTHDF1 in paraffin-embedded rat brain tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of YTHDF1 in paraffin-embedded mouse brain tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of YTHDF1 in paraffin-embedded human brain tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of YTHDF1 in paraffin-embedded mouse hippocampus tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of YTHDF1 in paraffin-embedded mouse brain tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of YTHDF1 in paraffin-embedded human brain tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) with a dilution of 1:1,000 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen, and tissues were counterstained with hematoxylin and mounted with DPX.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of YTHDF1 in 293 cells. The cells were fixed, permeabilized, and then stained with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of YTHDF1 in PC-12 cells. The cells were fixed, permeabilized, and then stained with YTHDF1 recombinant monoclonal antibody (Product # MA5-50793) at a dilution of 1 µg/mL (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at 4℃ for an hour, cells were stained with secondary antibody iFluor™ 488 conjugate-Goat anti-Rabbit IgG at a dilution of 1:1,000 for 30 minutes at 4℃. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

MA5-50793