10587-1-AP

antibody from Proteintech Group

Targeting: RBM15 OTT, OTT1

Provider product page for 10587-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [40]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

Submit

Product number: 10587-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#10587-1-AP, RRID:AB_2175757
Product name: RBM15 antibody
Antibody type: Polyclonal
Description: KD/KO validated RBM15 antibody (Cat. #10587-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RBM15 protein structure - 10587-1-AP shown in red.

Submitted references XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression.

Wang K, Bhattacharya A, Haratake N, Daimon T, Nakashoji A, Ozawa H, Peng B, Li W, Kufe D
Cell death & disease 2024 May 13;15(5):330

N6-methyladenosine-modified CircPSMA7 enhances bladder cancer malignancy through the miR-128-3p/MAPK1 axis.

Yi J, Ma X, Ying Y, Liu Z, Tang Y, Shu X, Sun J, Wu Y, Lu D, Wang X, Luo J, Liu B, Zheng X, Lin Y, Li J, Xie L
Cancer letters 2024 Mar 31;585:216613

Landscape of transcriptome-wide m(6)A modification in diabetic liver reveals rewiring of PI3K-Akt signaling after physical exercise.

Chen C, Jiang C, Lin T, Hu Y, Wu H, Xiang Q, Yang M, Wang S, Han X, Tao J
Acta physiologica (Oxford, England) 2024 Jun;240(6):e14154

BIRC5 facilitates cisplatin-chemoresistance in a m(6)A-dependent manner in ovarian cancer.

Fan Y, Pan Y, Jia L, Gu S, Liu B, Mei Z, Lv C, Huang H, Zhu G, Deng Q
Cancer medicine 2024 Jan;13(1):e6811

Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer.

Ying Y, Wu Y, Zhang F, Tang Y, Yi J, Ma X, Li J, Chen D, Wang X, Liu X, Liu B, Luo J, Zheng X, Xie L
Molecular cancer 2024 Apr 24;23(1):79

Comprehensive analyses of m6A RNA methylation patterns and related immune microenvironment in idiopathic pulmonary arterial hypertension.

Gao G, Chen A, Gong J, Lin W, Wu W, Mohammad Ismail Hajary S, Lian G, Luo L, Xie L
Frontiers in genetics 2023;14:1222368

RNA methylation pattern and immune microenvironment characteristics mediated by m6A regulator in ischemic stroke.

Jia K, Xia W, Su Q, Yang S, Zhang Y, Ni X, Su Z, Meng D
Frontiers in genetics 2023;14:1148510

RBFOX2 recognizes N(6)-methyladenosine to suppress transcription and block myeloid leukaemia differentiation.

Dou X, Xiao Y, Shen C, Wang K, Wu T, Liu C, Li Y, Yu X, Liu J, Dai Q, Pajdzik K, Ye C, Ge R, Gao B, Yu J, Sun S, Chen M, Chen J, He C
Nature cell biology 2023 Sep;25(9):1359-1368

FTO alleviates cerebral ischemia/reperfusion-induced neuroinflammation by decreasing cGAS mRNA stability in an m6A-dependent manner.

Yu Z, Zheng L, Geng Y, Zhang Y, Wang Y, You G, Cai M, Li M, Cheng X, Zan J
Cellular signalling 2023 Sep;109:110751

RBM15‑mediating MDR1 mRNA m(6)A methylation regulated by the TGF‑β signaling pathway in paclitaxel‑resistant ovarian cancer.

Yuan J, Guan W, Li X, Wang F, Liu H, Xu G
International journal of oncology 2023 Oct;63(4)

CSTF2 mediated mRNA N(6)-methyladenosine modification drives pancreatic ductal adenocarcinoma m(6)A subtypes.

Zheng Y, Li X, Deng S, Zhao H, Ye Y, Zhang S, Huang X, Bai R, Zhuang L, Zhou Q, Li M, Su J, Li R, Bao X, Zeng L, Chen R, Zheng J, Lin D, He C, Zhang J, Zuo Z
Nature communications 2023 Oct 10;14(1):6334

Identification of osteosarcoma m6A-related prognostic biomarkers using artificial intelligence: RBM15.

Jiang J, Qu H, Zhan X, Liu D, Liang T, Chen L, Huang S, Sun X, Chen J, Chen T, Li H, Yao Y, Liu C
Scientific reports 2023 Mar 31;13(1):5255

The differences in biological behavior and gene expression characteristics between pure and mixed early gastric signet ring cell carcinomas.

Wang X, Li X, Yang P, Qin X, Yu J, Cheng Y, Zhang G, Tang L
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver 2023 Jun;55(6):815-822

RBM15 suppresses hepatic insulin sensitivity of offspring of gestational diabetes mellitus mice via m6A-mediated regulation of CLDN4.

Fang J, Wu X, He J, Zhang H, Chen X, Zhang H, Novakovic B, Qi H, Yu X
Molecular medicine (Cambridge, Mass.) 2023 Feb 20;29(1):23

A lncRNA from the FTO locus acts as a suppressor of the m(6)A writer complex and p53 tumor suppression signaling.

Zhang J, Wei J, Sun R, Sheng H, Yin K, Pan Y, Jimenez R, Chen S, Cui XL, Zou Z, Yue Z, Emch MJ, Hawse JR, Wang L, He HH, Xia S, Han B, He C, Huang H
Molecular cell 2023 Aug 3;83(15):2692-2708.e7

m6A Regulatory Gene-Mediated Methylation Modification in Glioma Survival Prediction.

Zhang G, Zheng P, Lv Y, Shi Z, Shi F
Frontiers in genetics 2022;13:873764

Systematic analysis of the expression profile and prognostic significance of m6A regulators and PD-L1 in hepatocellular carcinoma.

Kong F, Wang K, Wang L
Discover oncology 2022 Nov 25;13(1):131

Analysis and identification of m(6)A RNA methylation regulators in metastatic osteosarcoma.

Huang H, Cui X, Qin X, Li K, Yan G, Lu D, Zheng M, Hu Z, Lei D, Lan N, Zheng L, Yuan Z, Zhu B, Zhao J
Molecular therapy. Nucleic acids 2022 Mar 8;27:577-592

Mettl3 promotes oxLDL-mediated inflammation through activating STAT1 signaling.

Li Z, Xu Q, Huangfu N, Chen X, Zhu J
Journal of clinical laboratory analysis 2022 Jan;36(1):e24019

YTHDC1 regulates distinct post-integration steps of HIV-1 replication and is important for viral infectivity.

N'Da Konan S, Ségéral E, Bejjani F, Bendoumou M, Ait Said M, Gallois-Montbrun S, Emiliani S
Retrovirology 2022 Jan 31;19(1):4

Cross talk between RNA modification writers and tumor development as a basis for guiding personalized therapy of gastric cancer.

Zhang S, Kuang G, Huang Y, Huang X, Wang W, Wang G
Human genomics 2022 Apr 22;16(1):14

Methylation and Expression of the Exercise-Related TLR1 Gene Is Associated With Low Grade Glioma Prognosis and Outcome.

Lu L, Hu Y, Wang C, Jiang F, Wu C
Frontiers in molecular biosciences 2021;8:747933

mTORC1 stimulates cell growth through SAM synthesis and m(6)A mRNA-dependent control of protein synthesis.

Villa E, Sahu U, O'Hara BP, Ali ES, Helmin KA, Asara JM, Gao P, Singer BD, Ben-Sahra I
Molecular cell 2021 May 20;81(10):2076-2093.e9

METTL3 regulates heterochromatin in mouse embryonic stem cells.

Xu W, Li J, He C, Wen J, Ma H, Rong B, Diao J, Wang L, Wang J, Wu F, Tan L, Shi YG, Shi Y, Shen H
Nature 2021 Mar;591(7849):317-321

N(6)-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles.

Wang X, Liu C, Zhang S, Yan H, Zhang L, Jiang A, Liu Y, Feng Y, Li D, Guo Y, Hu X, Lin Y, Bu P, Li D
Developmental cell 2021 Mar 8;56(5):702-715.e8

Integrated analysis of m(6)A mRNA methylation in rats with monocrotaline-induced pulmonary arterial hypertension.

Zeng Y, Huang T, Zuo W, Wang D, Xie Y, Wang X, Xiao Z, Chen Z, Liu Q, Liu N, Xiao Y
Aging 2021 Jul 26;13(14):18238-18256

RBM15 facilitates laryngeal squamous cell carcinoma progression by regulating TMBIM6 stability through IGF2BP3 dependent.

Wang X, Tian L, Li Y, Wang J, Yan B, Yang L, Li Q, Zhao R, Liu M, Wang P, Sun Y
Journal of experimental & clinical cancer research : CR 2021 Feb 26;40(1):80

The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation.

Coker H, Wei G, Moindrot B, Mohammed S, Nesterova T, Brockdorff N
Wellcome open research 2020;5:31

Expression and Prognostic Significance of m6A-Related Genes in Lung Adenocarcinoma.

Zhang Y, Liu X, Liu L, Li J, Hu Q, Sun R
Medical science monitor : international medical journal of experimental and clinical research 2020 Feb 22;26:e919644

RBM15 Modulates the Function of Chromatin Remodeling Factor BAF155 Through RNA Methylation in Developing Cortex.

Xie Y, Castro-Hernández R, Sokpor G, Pham L, Narayanan R, Rosenbusch J, Staiger JF, Tuoc T
Molecular neurobiology 2019 Nov;56(11):7305-7320

Splicing factor SF3B1K700E mutant dysregulates erythroid differentiation via aberrant alternative splicing of transcription factor TAL1.

Jin S, Su H, Tran NT, Song J, Lu SS, Li Y, Huang S, Abdel-Wahab O, Liu Y, Zhao X
PloS one 2017;12(5):e0175523

Cross-talk between PRMT1-mediated methylation and ubiquitylation on RBM15 controls RNA splicing.

Zhang L, Tran NT, Su H, Wang R, Lu Y, Tang H, Aoyagi S, Guo A, Khodadadi-Jamayran A, Zhou D, Qian K, Hricik T, Côté J, Han X, Zhou W, Laha S, Abdel-Wahab O, Levine RL, Raffel G, Liu Y, Chen D, Li H, Townes T, Wang H, Deng H, Zheng YG, Leslie C, Luo M, Zhao X
eLife 2015 Nov 17;4

A Pooled shRNA Screen Identifies Rbm15, Spen, and Wtap as Factors Required for Xist RNA-Mediated Silencing.

Moindrot B, Cerase A, Coker H, Masui O, Grijzenhout A, Pintacuda G, Schermelleh L, Nesterova TB, Brockdorff N
Cell reports 2015 Jul 28;12(4):562-72

Multiple regions of Kaposi's sarcoma-associated herpesvirus ORF59 RNA are required for its expression mediated by viral ORF57 and cellular RBM15.

Massimelli MJ, Majerciak V, Kang JG, Liewehr DJ, Steinberg SM, Zheng ZM
Viruses 2015 Feb 3;7(2):496-510

Attenuation of the suppressive activity of cellular splicing factor SRSF3 by Kaposi sarcoma-associated herpesvirus ORF57 protein is required for RNA splicing.

Majerciak V, Lu M, Li X, Zheng ZM
RNA (New York, N.Y.) 2014 Nov;20(11):1747-58

Rbm15-Mkl1 interacts with the Setd1b histone H3-Lys4 methyltransferase via a SPOC domain that is required for cytokine-independent proliferation.

Lee JH, Skalnik DG
PloS one 2012;7(8):e42965

Kaposi's sarcoma-associated herpesvirus ORF57 interacts with cellular RNA export cofactors RBM15 and OTT3 to promote expression of viral ORF59.

Majerciak V, Uranishi H, Kruhlak M, Pilkington GR, Massimelli MJ, Bear J, Pavlakis GN, Felber BK, Zheng ZM
Journal of virology 2011 Feb;85(4):1528-40

Requirement of UAP56, URH49, RBM15, and OTT3 in the expression of Kaposi sarcoma-associated herpesvirus ORF57.

Majerciak V, Deng M, Zheng ZM
Virology 2010 Nov 25;407(2):206-12

The RNA-binding motif protein 15B (RBM15B/OTT3) acts as cofactor of the nuclear export receptor NXF1.

Uranishi H, Zolotukhin AS, Lindtner S, Warming S, Zhang GM, Bear J, Copeland NG, Jenkins NA, Pavlakis GN, Felber BK
The Journal of biological chemistry 2009 Sep 18;284(38):26106-16

Nuclear export factor RBM15 facilitates the access of DBP5 to mRNA.

Zolotukhin AS, Uranishi H, Lindtner S, Bear J, Pavlakis GN, Felber BK
Nucleic acids research 2009 Nov;37(21):7151-62

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10587-1-AP(RBM15 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using RBM15 antibody 10587-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human breast cancer using 10587-1-AP(RBM15 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue