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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [10]
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- Product number
- TA500671 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500671, RRID:AB_2138755
- Product name
- Anti-LTA4H (Leukotriene A4 hydrolase) mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)
- Antibody type
- Monoclonal
- Description
- Anti-LTA4H (Leukotriene A4 hydrolase) mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- LTA4H
- Isotype
- IgG
- Antibody clone number
- OTI2A9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references An aberrant leukotriene A4 hydrolase-proline-glycine-proline pathway in the pathogenesis of chronic obstructive pulmonary disease.
Wells JM, O'Reilly PJ, Szul T, Sullivan DI, Handley G, Garrett C, McNicholas CM, Roda MA, Miller BE, Tal-Singer R, Gaggar A, Rennard SI, Jackson PL, Blalock JE
American journal of respiratory and critical care medicine 2014 Jul 1;190(1):51-61
American journal of respiratory and critical care medicine 2014 Jul 1;190(1):51-61
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-LTA4H monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LTA4H (RC207617, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LTA4H.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-LTA4H mouse monoclonal antibody (TA500671) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LTA4H(RC207617).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of LTA4H by using TrueMab monoclonal anti-LTA4H antibody (Negative control: IP without adding anti-LTA4H antibody). For each experiment, 500ul of DDK tagged LTA4H overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Carcinoma of prostate tissue using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded breast tissue within the normal limits using anti-LTA4H mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of ovary tissue using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded thyroid tissue within the normal limits using anti-LTA4Hmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500671, Dilution 1:50)
- Validation comment
- IHC
