16448-1-AP

antibody from Proteintech Group

Targeting: DEK D6S231E

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

16448-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16448-1-AP, RRID:AB_2092097

Product name

DEK antibody

Antibody type

Polyclonal

Description

KD/KO validated DEK antibody (Cat. #16448-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Exosomal DEK removes chemoradiotherapy resistance by triggering quiescence exit of breast cancer stem cells.

Yang YS, Jia XZ, Lu QY, Cai SL, Huang XT, Yang SH, Wood C, Wang YH, Zhou JJ, Chen YD, Yang JS, Yang WJ
Oncogene 2022 Apr;41(18):2624-2637

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Nuclear DEK preserves hematopoietic stem cells potential via NCoR1/HDAC3-Akt1/2-mTOR axis.

Chen Z, Huo D, Li L, Liu Z, Li Z, Xu S, Huang Y, Wu W, Zhou C, Liu Y, Kuang M, Wu F, Li H, Qian P, Song G, Wu X, Chen J, Hou Y
The Journal of experimental medicine 2021 May 3;218(5)

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DEK is highly expressed in breast cancer and is associated with malignant phenotype and progression.

Yang MQ, Bai LL, Wang Z, Lei L, Zheng YW, Li ZH, Huang WJ, Liu CC, Xu HT
Oncology letters 2021 Jun;21(6):440

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ATRX limits the accessibility of histone H3-occupied HSV genomes during lytic infection.

Cabral JM, Cushman CH, Sodroski CN, Knipe DM
PLoS pathogens 2021 Apr;17(4):e1009567

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Loss of DEK Expression Induces Alzheimer's Disease Phenotypes in Differentiated SH-SY5Y Cells.

Greene AN, Parks LG, Solomon MB, Privette Vinnedge LM
Frontiers in molecular neuroscience 2020;13:594319

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Dek Modulates Global Intron Retention during Muscle Stem Cells Quiescence Exit.

Yue L, Wan R, Luan S, Zeng W, Cheung TH
Developmental cell 2020 Jun 22;53(6):661-676.e6

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DEK Expression in Breast Cancer Cells Leads to the Alternative Activation of Tumor Associated Macrophages.

Pease NA, Shephard MS, Sertorio M, Waltz SE, Vinnedge LMP
Cancers 2020 Jul 17;12(7)

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Protocol for Isolation and Characterization of In Situ Fixed Quiescent Muscle Stem Cells.

Yue L, Cheung TH
STAR protocols 2020 Dec 18;1(3):100128

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DEK promotes the proliferation and invasion of lung cancers and indicates poor prognosis in lung adenocarcinomas.

Yang MQ, Bai LL, Lei L, Zheng YW, Wang Z, Li ZH, Liu CC, Huang WJ, Xu HT
Oncology reports 2020 Apr;43(4):1338-1348

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DEK Is a Potential Biomarker Associated with Malignant Phenotype in Gastric Cancer Tissues and Plasma.

Lee KF, Tsai MM, Tsai CY, Huang CG, Ou YH, Hsieh CC, Hsieh HL, Wang CS, Lin KH
International journal of molecular sciences 2019 Nov 13;20(22)

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Overexpression of miR-489 derails mammary hierarchy structure and inhibits HER2/neu-induced tumorigenesis.

Patel Y, Soni M, Awgulewitsch A, Kern MJ, Liu S, Shah N, Singh UP, Chen H
Oncogene 2019 Jan;38(3):445-453

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Argininosuccinate synthetase 1 contributes to gastric cancer invasion and progression by modulating autophagy.

Tsai CY, Chi HC, Chi LM, Yang HY, Tsai MM, Lee KF, Huang HW, Chou LF, Cheng AJ, Yang CW, Wang CS, Lin KH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2018 May;32(5):2601-2614

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Dek overexpression in murine epithelia increases overt esophageal squamous cell carcinoma incidence.

Matrka MC, Cimperman KA, Haas SR, Guasch G, Ehrman LA, Waclaw RR, Komurov K, Lane A, Wikenheiser-Brokamp KA, Wells SI
PLoS genetics 2018 Mar;14(3):e1007227

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Oncogene DEK is highly expressed in lung cancerous tissues and positively regulates cell proliferation as well as invasion.

Zhou QC, Deng XF, Yang J, Jiang H, Qiao MX, Liu HH, Qian Z, Hou LL, Hu HG
Oncology letters 2018 Jun;15(6):8573-8581

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SPOP promotes ATF2 ubiquitination and degradation to suppress prostate cancer progression.

Ma J, Chang K, Peng J, Shi Q, Gan H, Gao K, Feng K, Xu F, Zhang H, Dai B, Zhu Y, Shi G, Shen Y, Zhu Y, Qin X, Li Y, Zhang P, Ye D, Wang C
Journal of experimental & clinical cancer research : CR 2018 Jul 11;37(1):145

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Neuroanatomical Distribution of DEK Protein in Corticolimbic Circuits Associated with Learning and Memory in Adult Male and Female Mice.

Ghisays V, Nguyen ET, Streicher J, Pease NA, Fitzgerald M, Estrada CM, Franco-Villanueva A, Privette Vinnedge L, Solomon MB
Neuroscience 2018 Feb 10;371:254-267

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DEK is required for homologous recombination repair of DNA breaks.

Smith EA, Gole B, Willis NA, Soria R, Starnes LM, Krumpelbeck EF, Jegga AG, Ali AM, Guo H, Meetei AR, Andreassen PR, Kappes F, Vinnedge LM, Daniel JA, Scully R, Wiesmüller L, Wells SI
Scientific reports 2017 Mar 20;7:44662

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Fbw7 regulates apoptosis in activated B-cell like diffuse large B-cell lymphoma by targeting Stat3 for ubiquitylation and degradation.

Yao S, Xu F, Chen Y, Ge Y, Zhang F, Huang H, Li L, Lin D, Luo X, Xu J, Luo D, Zhu X, Liu Y
Journal of experimental & clinical cancer research : CR 2017 Jan 10;36(1):10

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Regulation of DEK expression by AP-2α and methylation level of DEK promoter in hepatocellular carcinoma.

Qiao MX, Li C, Zhang AQ, Hou LL, Yang J, Hu HG
Oncology reports 2016 Oct;36(4):2382-90

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DEK over-expression promotes mitotic defects and micronucleus formation.

Matrka MC, Hennigan RF, Kappes F, DeLay ML, Lambert PF, Aronow BJ, Wells SI
Cell cycle (Georgetown, Tex.) 2015;14(24):3939-53

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Hira-mediated H3.3 incorporation is required for DNA replication and ribosomal RNA transcription in the mouse zygote.

Lin CJ, Koh FM, Wong P, Conti M, Ramalho-Santos M
Developmental cell 2014 Aug 11;30(3):268-79

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DEK depletion negatively regulates Rho/ROCK/MLC pathway in non-small cell lung cancer.

Wang J, Sun L, Yang M, Luo W, Gao Y, Liu Z, Qiu X, Wang E
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Jul;61(7):510-21

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The histone chaperone Spt6 is required for activation-induced cytidine deaminase target determination through H3K4me3 regulation.

Begum NA, Stanlie A, Nakata M, Akiyama H, Honjo T
The Journal of biological chemistry 2012 Sep 21;287(39):32415-29

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Maintenance of muscle stem-cell quiescence by microRNA-489.

Cheung TH, Quach NL, Charville GW, Liu L, Park L, Edalati A, Yoo B, Hoang P, Rando TA
Nature 2012 Feb 23;482(7386):524-8

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16448-1-AP(DEK antibody) at dilution of 1:800

Sample type

cell line

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using DEK antibody 16448-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

cell line

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16448-1-AP(DEK antibody) at dilution of 1:100 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 16448-1-AP(DEK antibody) at dilution of 1:100 (under 40x lens)

Sample type

tissue