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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-63488 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DEK Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KFRNAMLKSI CEVLDLERSG VNSELVKRIL NFLMHPKPSG KPLPKSKKTC SKGSKKERNS SGMARKAKRT KCPEI
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DEK in Lane 1: Marker (kDa) 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251 MG; Lane 4: Human plasma; Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using a DEK Polyclonal Antibody (Product # PA5-63488).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DEK Polyclonal Antibody (Product # PA5-63488) and a 50 kDa band corresponding to DEK was observed in all the tested cell lines and the expression was lower in MCF 10A when compared to MDA-MB-231. Modified whole cell lysates (1% SDS) (30 µg lysate) of HeLa (Lane 1), PANC-1 (Lane 2), IMR-32 (Lane 3), PC-3 (Lane 4), Jurkat (Lane 5), MCF 10A (Lane 6) and MDA-MB-231 (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (0.4 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of DEK in human cell line RH-30 using a DEK Polyclonal Antibody (Product # PA5-63488) shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of DEK in human stomach tissue shows moderate nuclear positivity in glandular cells. Samples were probed using a DEK Polyclonal Antibody (Product # PA5-63488).
