51002-1-AP

antibody from Proteintech Group

Targeting: IST1 CHMP8, KIAA0174

Provider product page for 51002-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [29]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [1]

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Product number: 51002-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#51002-1-AP, RRID:AB_2130516
Product name: hIST1 antibody
Antibody type: Polyclonal
Description: KD/KO validated hIST1 antibody (Cat. #51002-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

IST1 protein structure - 51002-1-AP shown in red.

Submitted references The ATG8 E3-like ligases sense lysosomal damage and initiate ESCRT-mediated membrane repair.

Corkery DP, Wijayatunga D, Feron BKL, Herzog LK, Knyazeva A, Wu YW
The EMBO journal 2026 Feb;45(3):930-952

ATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains.

Reinders M, Kravic B, Gahlot P, Koska S, van den Boom J, Schulze N, Levantovsky S, Kleine S, Kaiser M, Kulathu Y, Behrends C, Meyer H
The EMBO journal 2025 Sep;44(18):5086-5111

Loss of CHMP2A implicates an ordered assembly of ESCRT-III proteins during cytokinetic abscission.

Kamenetsky N, Nachmias D, Khan S, Avinoam O, Hazan I, Upcher A, Elia N
Molecular biology of the cell 2025 Nov 1;36(11):ar143

Euphorbia factor L2 alleviated gouty inflammation by specifically suppressing both the priming and activation of NLRP3 inflammasome.

Li Y, Zhuang Y, Chen Y, Wang G, Tang Z, Zhong Y, Zhang Y, Wu L, Ji X, Zhang Q, Pan B, Luo Y
International immunopharmacology 2024 Sep 10;138:112598

Cigarette smoking-related OLC1 overexpression associated with poor prognosis in bladder urothelial carcinoma.

Chen H, Cai Z, Dong X, Chen W, Cao C, Zheng S, Wu J, Zhong D, Cheng S, Gao Y, Shou J
Life sciences 2024 Aug 15;351:122821

The human AAA-ATPase VPS4A isoform and its co-factor VTA1 have a unique function in regulating mammalian cytokinesis abscission.

Dvilansky I, Altaras Y, Kamenetsky N, Nachmias D, Elia N
PLoS biology 2024 Apr;22(4):e3002327

Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH.

Gahlot P, Kravic B, Rota G, van den Boom J, Levantovsky S, Schulze N, Maspero E, Polo S, Behrends C, Meyer H
Molecular cell 2024 Apr 18;84(8):1556-1569.e10

Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport (ESCRT) protein.

Shen W, Liu C, Hu Y, Ding Q, Feng J, Liu Z, Kong X
Virologica Sinica 2023 Jun;38(3):448-458

V. cholerae MakA is a cholesterol-binding pore-forming toxin that induces non-canonical autophagy.

Jia X, Knyazeva A, Zhang Y, Castro-Gonzalez S, Nakamura S, Carlson LA, Yoshimori T, Corkery DP, Wu YW
The Journal of cell biology 2022 Dec 5;221(12)

CDK1 controls CHMP7-dependent nuclear envelope reformation.

Gatta AT, Olmos Y, Stoten CL, Chen Q, Rosenthal PB, Carlton JG
eLife 2021 Jul 21;10

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

Merigliano C, Burla R, La Torre M, Del Giudice S, Teo H, Liew CW, Chojnowski A, Goh WI, Olmos Y, Maccaroni K, Giubettini M, Chiolo I, Carlton JG, Raimondo D, Vernì F, Stewart CL, Rhodes D, Wright GD, Burke BE, Saggio I
PLoS genetics 2021 Aug;17(8):e1009757

ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic.

Connell JW, Allison RJ, Rodger CE, Pearson G, Zlamalova E, Reid E
Cellular and molecular life sciences : CMLS 2020 Jul;77(13):2641-2658

De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment.

Rodger C, Flex E, Allison RJ, Sanchis-Juan A, Hasenahuer MA, Cecchetti S, French CE, Edgar JR, Carpentieri G, Ciolfi A, Pantaleoni F, Bruselles A, Genomics England Research Consortium, Onesimo R, Zampino G, Marcon F, Siniscalchi E, Lees M, Krishnakumar D, McCann E, Yosifova D, Jarvis J, Kruer MC, Marks W, Campbell J, Allen LE, Gustincich S, Raymond FL, Tartaglia M, Reid E
American journal of human genetics 2020 Dec 3;107(6):1129-1148

Studying the Spatial Organization of ESCRTs in Cytokinetic Abscission Using the High-Resolution Imaging Techniques SIM and Cryo-SXT.

Adar-Levor S, Goliand I, Elbaum M, Elia N
Methods in molecular biology (Clifton, N.J.) 2019;1998:129-148

Regulation of OLC1 protein expression by the anaphase-promoting complex.

Zhang X, Chen W, Yin N, Dong L, Fu M, Zhan Q, Tong T
Oncology letters 2019 Mar;17(3):2639-2646

Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Liu S, Kwon M, Mannino M, Yang N, Renda F, Khodjakov A, Pellman D
Nature 2018 Sep;561(7724):551-555

VPS4 is a dynamic component of the centrosome that regulates centrosome localization of γ-tubulin, centriolar satellite stability and ciliogenesis.

Ott C, Nachmias D, Adar S, Jarnik M, Sherman S, Birnbaum RY, Lippincott-Schwartz J, Elia N
Scientific reports 2018 Feb 20;8(1):3353

Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.

Goliand I, Adar-Levor S, Segal I, Nachmias D, Dadosh T, Kozlov MM, Elia N
Cell reports 2018 Aug 14;24(7):1756-1764

Defects in ER-endosome contacts impact lysosome function in hereditary spastic paraplegia.

Allison R, Edgar JR, Pearson G, Rizo T, Newton T, Günther S, Berner F, Hague J, Connell JW, Winkler J, Lippincott-Schwartz J, Beetz C, Winner B, Reid E
The Journal of cell biology 2017 May 1;216(5):1337-1355

An Automated Image Analysis System to Quantify Endosomal Tubulation.

Newton TM, Reid E
PloS one 2016;11(12):e0168294

ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.

Christ L, Wenzel EM, Liestøl K, Raiborg C, Campsteijn C, Stenmark H
The Journal of cell biology 2016 Feb 29;212(5):499-513

Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Vietri M, Schink KO, Campsteijn C, Wegner CS, Schultz SW, Christ L, Thoresen SB, Brech A, Raiborg C, Stenmark H
Nature 2015 Jun 11;522(7555):231-5

Over-expression of the overexpressed in lung cancer-1 is associated with poor prognosis in colorectal cancer.

Liu J, Song H, Yao L, Liu Y, Zhang Y, Zhao H, Ji H, Wang Y
Anticancer research 2014 Jan;34(1):367-72

Over-expression of the overexpressed in lung cancer 1 is associated with poor prognosis in epithelial ovarian cancer.

Jia C, Li X, Sun H, Sui L
Journal of surgical oncology 2013 Jun;107(8):847-52

An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.

Allison R, Lumb JH, Fassier C, Connell JW, Ten Martin D, Seaman MN, Hazan J, Reid E
The Journal of cell biology 2013 Aug 5;202(3):527-43

ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.

Hadders MA, Agromayor M, Obita T, Perisic O, Caballe A, Kloc M, Lamers MH, Williams RL, Martin-Serrano J
Proceedings of the National Academy of Sciences of the United States of America 2012 Oct 23;109(43):17424-9

The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain.

Agromayor M, Soler N, Caballe A, Kueck T, Freund SM, Allen MD, Bycroft M, Perisic O, Ye Y, McDonald B, Scheel H, Hofmann K, Neil SJ, Martin-Serrano J, Williams RL
Structure (London, England : 1993) 2012 Mar 7;20(3):414-28

Cigarette smoke suppresses the ubiquitin-dependent degradation of OLC1.

Zhang X, Xiao T, Cheng S, Tong T, Gao Y
Biochemical and biophysical research communications 2011 Apr 22;407(4):753-7

Essential role of hIST1 in cytokinesis.

Agromayor M, Carlton JG, Phelan JP, Matthews DR, Carlin LM, Ameer-Beg S, Bowers K, Martin-Serrano J
Molecular biology of the cell 2009 Mar;20(5):1374-87

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 51002-1-AP(hIST1 antibody) at dilution of 1:300
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using KIAA0174 antibody 51002-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human lung cancer using 51002-1-AP(hIST1 antibody) at dilution of 1:200 (under 40x lens)
Sample type: tissue