Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 39107 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Histone H2AK5ac Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- Conc. Not Determined
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Histone H2A acetyl Lys5 pAb tested by Western blot. HeLa acid extract (5 µg per lane) was probed with Histone H2A acetyl Lys5 polyclonal antibody at a 1:2,000 dilution. Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of Histone H2AK5ac by immunofluorescence. U2OS cells were stained with H2AK5ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK5ac antibody staining. Right panel: merge.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Specificity data:. Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys5 pAb for acetyl Lys5 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:2,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row. Lane 1: Peptide acetylated at Lys5 of H2A. Lane 2: Unmodified Lys5 peptide. Lane 3: Peptide acetylated at Lys9 of H2A. Lane 4: Unmodified Lys9 peptide.
