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LearnPA5-24822
antibody from Invitrogen Antibodies
Targeting: H2AC11
H2A.1b, H2A/p, H2AFP, HIST1H2AG, pH2A/f
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- PA5-24822 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HIST1H2AG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse and rat based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references LINC00842 inactivates transcription co-regulator PGC-1α to promote pancreatic cancer malignancy through metabolic remodelling.
Huang X, Pan L, Zuo Z, Li M, Zeng L, Li R, Ye Y, Zhang J, Wu G, Bai R, Zhuang L, Wei L, Zheng Y, Su J, Deng J, Deng S, Zhang S, Zhu S, Che X, Wang C, Wu C, Chen R, Lin D, Zheng J
Nature communications 2021 Jun 22;12(1):3830
Nature communications 2021 Jun 22;12(1):3830
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a HIST1H2AL polyclonal antibody (Product # PA5-24822) in HL-60, HepG2, K562, CEM, MCF-7 cell lysates (35 µg per lane).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a HIST1H2AL polyclonal antibody (Product # PA5-24822) in HL-60, HepG2, K562, CEM, MCF-7 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HIST1H2AG was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with HIST1H2AG Rabbit Polyclonal Antibody (Product # PA5-24822) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human lung carcinoma using a HIST1H2AL polyclonal antibody (Product # PA5-24822), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of HepG2 cells using a HIST1H2AL polyclonal antibody (Product # PA5-24822) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Enrichment of endogenous HIST1H2AG protein at specific gene loci using Anti-HIST1H2AG Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-HIST1H2AG Rabbit Polyclonal Antibody (Product # PA5-24822, 3 µg) on sheared chromatin from 2 million HeLa cells using the MAGnify ChIP system kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs for the promoters of c-Fos, ALDOA,GAPDH used as positive and MYOD, SAT2 satellite repeats, SAT Alpha used as negative target genes/binding sites. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
