H00010325-M01

antibody from Abnova Corporation

Targeting: RRAGB

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00010325-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00010325-M01, RRID:AB_463884

Product name

RRAGB monoclonal antibody (M01), clone 2B8

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant RRAGB.

Antigen sequence

MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLP
NTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTR
RLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMEN
YFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQ
SCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFK
EREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIV
YQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFL
VISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAA
SFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIP
SAATLINIRNARKHFEKLERVDGPKQCLLMR


Isotype

IgG

Antibody clone number

2B8

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RRAGB expression in transfected 293T cell line by RRAGB monoclonal antibody (M01), clone 2B8.Lane 1: RRAGB transfected lysate(43.3 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged RRAGB is 1 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol