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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-96453 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WNT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SWWYMRATGG SSRVMCDNVP GLVSSQRQLC HRHPDVMRAI SQGVAEWTAE CQHQFRQHRW NCNTLDRDHS LFGRVLLRSS RESAFVYAIS SAGVVFAITR ACSQGEVKSC SCDPKKMGSA KDSKGIFDWG GCSDNIDYGI KFARAFVDAK ERKGKDARAL MNLHNNRAGR KAVKRFLKQE CKCHGVSGSC TLRTCWLAMA DFRKTGDYLW RKYNGAIQVV MNQDGTGFTV ANERFKKPTK NDLVYFENSP DYCIRDREAG SLGTAGRVCN LTSRGMDSCE VMCCGRGYDT SHVTRMTKCG CKFHWCCAVR CQDCLEALDV HTCKAPKNAD WTTAT; Positive Samples: SKOV3; Cellular Location: extracellular matrix, extracellular space
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.03 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of WNT2 in extracts of SKOV3 cells using WNT2 Polyclonal Antibody (Product # PA5-96453) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of WNT2 in paraffin-embedded rat brain using WNT2 Polyclonal Antibody (Product # PA5-96453) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of WNT2 in paraffin-embedded human liver damage using WNT2 Polyclonal Antibody (Product # PA5-96453) at a dilution of 1:200.
