HPA024377

antibody from Atlas Antibodies

Targeting: IKZF3 Aiolos, ZNFN1A3

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA024377

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA024377, RRID:AB_1859477

Product name

Anti-IKZF3

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AVLNDYSLTKSHEMENVDSGEGPANEDEDIGDDSM
KVKDEYSERDENVLKSEPMGNAEEPEIPYSYSREY
NEYENIKLERHVVSFDSSRPTSGKMNCDVCGLSCI
SFNVL


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human tonsil and cerebral cortex tissues using Anti-IKZF3 antibody. Corresponding IKZF3 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human tonsil shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex shows low expression as expected.

Sample type

HUMAN