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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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- Product number
- PA5-23269 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKZF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide using a IkZF3, Zinc finger protein Aiolos polyclonal antibody (Product # PA5-23269) at 0.25 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKZF3 in Jurkat cells and human tonsil. Samples were incubated in IKZF3 polyclonal antibody (Product # PA5-23269) using a dilution of 2 µg/mL followed by an anti-rabbit HRP secondary antibody. Separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. Detection: chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKZF3 in human spleen lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in IKZF3 polyclonal antibody (Product # PA5-23269) using a dilution of 0.25 µg/mL.
