R31028

antibody from NSJ Bioreagents

Targeting: MYBL2 B-MYB, BMYB

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31028

Provider

NSJ Bioreagents

Product name

MYBL2 Antibody / B-Myb

Antibody type

Polyclonal

Description

This highly specific MYBL2 antibody is suitable for use in Western blot/Immunohistochemistry applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the MYBL2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing with MYBL2 antibody; Lane 1: rat spleen; 2: (r) thymus; 3: (r) brain; 4: human HeLa; 5: (h) COLO320; 6: (h) MCF-7 cell lysate. Expected size ~79KD

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing with MYBL2 antibody and recombinant protein, 47KD with tag; Lane 1: Recombinant protein at 10ng; 2: 5ng; 3: 2.5ng

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human Raji cell lysate with MYBL2 antibody. Predicted molecular weight: ~79 kDa, may also be observed at 100~110 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

ICC testing of MYBL2 antibody and A549 cells

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: MYBL2 antibody testing of human tonsil tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: MYBL2 antibody testing of rat brain tissue. HIER: steamed with pH6 citrate buffer.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse liver tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat liver tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human tonsil tissue with MYBL2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.