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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- AF-381-PB - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal G-CSFR/CD114 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human G-CSF R/CD114 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) G-CSF and rhGM-CSF R alpha is observed.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Most purported antibodies to the human granulocyte colony-stimulating factor receptor are not specific.
The Integrin alpha9beta1 contributes to granulopoiesis by enhancing granulocyte colony-stimulating factor receptor signaling.
deBruin C, Lincoln P, Hartley C, Shehabeldin A, Van G, Szilvassy SJ
Experimental hematology 2010 Nov;38(11):1022-35
Experimental hematology 2010 Nov;38(11):1022-35
The Integrin alpha9beta1 contributes to granulopoiesis by enhancing granulocyte colony-stimulating factor receptor signaling.
Chen C, Huang X, Atakilit A, Zhu QS, Corey SJ, Sheppard D
Immunity 2006 Dec;25(6):895-906
Immunity 2006 Dec;25(6):895-906
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human G-CSF R/CD114 by Western Blot. Western blot shows lysates of AML-193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human G-CSF R/CD114 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-381-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for G-CSF R/CD114 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions using Immunoblot Buffer Group 1.
