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- References [2]
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- Western blot [1]
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- Product number
- MAB3811 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal G-CSFR/CD114 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human G-CSF R/CD114 in ELISAs and Western blots. In sandwich Immunoassays, no cross-reactivity with recombinant human (rh) G-CSF, rhGM-CSF R beta , rhGM-CSF R, or recombinant mouse G-CSF is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references A Novel Germline Variant in CSF3R Reduces N-Glycosylation and Exerts Potent Oncogenic Effects in Leukemia.
Most purported antibodies to the human granulocyte colony-stimulating factor receptor are not specific.
Spiciarich DR, Oh ST, Foley A, Hughes SB, Mauro MJ, Abdel-Wahab O, Press RD, Viner R, Thompson SL, Chen Q, Azadi P, Bertozzi CR, Maxson JE
Cancer research 2018 Dec 15;78(24):6762-6770
Cancer research 2018 Dec 15;78(24):6762-6770
Most purported antibodies to the human granulocyte colony-stimulating factor receptor are not specific.
deBruin C, Lincoln P, Hartley C, Shehabeldin A, Van G, Szilvassy SJ
Experimental hematology 2010 Nov;38(11):1022-35
Experimental hematology 2010 Nov;38(11):1022-35
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human G-CSF R/CD114 by Western Blot. Western blot shows lysates of AML-193 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human G-CSF R/CD114 Monoclonal Antibody (Catalog # MAB3811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for G-CSF R/ CD114 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
