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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF6039 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse G-CSFR/CD114 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse G-CSF R/CD114 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GM-CSF R alpha and recombinant mouse GM-CSF R alpha is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P40223- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse G-CSF R/CD114 by Western Blot. Western blot shows lysates of ST-2 mouse bone marrow-derived stromal cell line. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse G-CSF R/CD114 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6039) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for G-CSF R/CD114 at approximately 110 and 112 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
