Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 46-1149-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD114 (G-CSFR) Monoclonal Antibody (LMM741), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This LMM741 monoclonal antibody reacts with human CD114, which is also known as granulocyte colony-stimulating factor receptor (G-CSFR). A type I hematopoietin cytokine receptor, CD114 is expressed on granulocytes such as neutrophils, myeloid cells, monocytes, and leukemic cells. This receptor is absent from lymphocytes, erythrocytes, and eosinophils. CD114 can also be detected on non-hematopoietic cells, including neurons, endothelial cells, and cardiomyocytes. Studies demonstrate that expression of CD114 can be downregulated by LPS, TNF, phorbol ester, and G-CSF itself. Binding of G-CSF binding induces homodimerization of the receptor that activates signaling transduction (e.g., the JAK/STAT and Erk1/2 pathways) to mediate cell proliferation, survival, and differentiation. Applications Reported: This LMM741 antibody has been reported for use in flow cytometric analysis. Applications Tested: This LMM741 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- LMM741
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments.
Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis.
Src kinases in G-CSF receptor signaling.
Endotoxin down-modulates granulocyte colony-stimulating factor receptor (CD114) on human neutrophils.
Expression and dynamic modulation of the human granulocyte colony-stimulating factor receptor in immature and differentiated myeloid cells.
Gibbs KD Jr, Gilbert PM, Sachs K, Zhao F, Blau HM, Weissman IL, Nolan GP, Majeti R
Blood 2011 Apr 21;117(16):4226-33
Blood 2011 Apr 21;117(16):4226-33
Granulocyte colony-stimulating factor: molecular mechanisms of action during steady state and 'emergency' hematopoiesis.
Panopoulos AD, Watowich SS
Cytokine 2008 Jun;42(3):277-88
Cytokine 2008 Jun;42(3):277-88
Src kinases in G-CSF receptor signaling.
Sampson M, Zhu QS, Corey SJ
Frontiers in bioscience : a journal and virtual library 2007 Jan 1;12:1463-74
Frontiers in bioscience : a journal and virtual library 2007 Jan 1;12:1463-74
Endotoxin down-modulates granulocyte colony-stimulating factor receptor (CD114) on human neutrophils.
Hollenstein U, Homoncik M, Stohlawetz PJ, Marsik C, Sieder A, Eichler HG, Jilma B
The Journal of infectious diseases 2000 Jul;182(1):343-6
The Journal of infectious diseases 2000 Jul;182(1):343-6
Expression and dynamic modulation of the human granulocyte colony-stimulating factor receptor in immature and differentiated myeloid cells.
Khwaja A, Carver J, Jones HM, Paterson D, Linch DC
British journal of haematology 1993 Oct;85(2):254-9
British journal of haematology 1993 Oct;85(2):254-9
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD114 (G-CSFR) PerCP-eFluor® 710. Cells in the lymphocyte (blue histogram), monocyte (orange histogram), and granulocyte (purple histogram) gates were used for analysis.