Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP2-61696 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal G-CSFR/CD114 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G-CSF R/CD114 Antibody (8F8B12) [NBP2-61696] - Analysis using CD114 mAb against human CD114 (AA: extra 25-187) recombinant protein. (Expected MW is 46.5 kDa)
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G-CSF R/CD114 Antibody (8F8B12) [NBP2-61696] - Analysis using CD114 mouse mAb against A431 (1), K562 (2), THP-1 (3), MOLT4 (4), and Jurkat (5) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: G-CSF R/CD114 Antibody (8F8B12) [NBP2-61696] - Analysis using CD114 mAb against HEK293 (1) and CD114 (AA: extra 25-187)-hIgGFc transfected HEK293 (2) cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: G-CSF R/CD114 Antibody (8F8B12) [NBP2-61696] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: G-CSF R/CD114 Antibody (8F8B12) [NBP2-61696] - Analysis of paraffin-embedded bladder cancer tissues using CD114 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: G-CSF R/CD114 Antibody (8F8B12) [NBP2-61696] - Analysis of Ramos cells using CD114 mouse mAb (green) and negative control (red).
