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- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
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- Product number
- MAB381 - Provider product page
- Provider
- R&D Systems
- Product name
- Human G-CSFR/CD114 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human G-CSF R/CD114 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) GM-CSF R alpha , rhGM-CSF R beta , or rhM-CSF R is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q99062- Isotype
- IgG
- Antibody clone number
- 38660
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Most purported antibodies to the human granulocyte colony-stimulating factor receptor are not specific.
Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
deBruin C, Lincoln P, Hartley C, Shehabeldin A, Van G, Szilvassy SJ
Experimental hematology 2010 Nov;38(11):1022-35
Experimental hematology 2010 Nov;38(11):1022-35
Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
Shao H, Xu X, Jing N, Tweardy DJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 1;176(5):2933-41
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 1;176(5):2933-41
Unique structural determinants for Stat3 recruitment and activation by the granulocyte colony-stimulating factor receptor at phosphotyrosine ligands 704 and 744.
Shao H, Xu X, Jing N, Tweardy DJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 1;176(5):2933-41
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 1;176(5):2933-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human G-CSF R/CD114 by Western Blot. Western blot shows lysates of AML-193 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human G-CSF R/CD114 Monoclonal Antibody (Catalog # MAB381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for G-CSF R/CD114 at approximately 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
