LS-C287372

antibody from LSBio

Targeting: HUWE1 Ib772, KIAA0312, UREB1

Western blot (Enhanced validation)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C287372

Provider

LSBio

Product name

HUWE1 / ARFBP1 Antibody (aa2250-2300) LS-C287372

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

Store at 2-8°C for up to one year.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Ureb1/Lasu1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), and HeLa (50 ug) cells. Antibodies: Affinity purified rabbit anti-Ureb1/Lasu1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Mouse Ureb1/Lasu1 by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Ureb1/Lasu1 antibody used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of Human Lasu1/Ureb1 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 used at a dilution of 1:1000 (1 ug/ml). Detection: DAB.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection of human Lasu1/Ureb1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB.