ABIN499331

antibody from antibodies-online

Targeting: HUWE1 Ib772, KIAA0312, UREB1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN499331

Provider

antibodies-online

Product name

anti-HECT, UBA and WWE Domain Containing 1, E3 Ubiquitin Protein Ligase (HUWE1) (C-Term) antibody

Antibody type

Polyclonal

Antigen

A 19 amino acid peptide from near the carboxy terminus of Human ARF-BP1 (AP30078CP-N)

Description

Affinity chromatography purified via peptide column

Reactivity

Human, Mouse, Rat

Host

Rabbit

Epitope

C-Term

Isotype

IgG

Vial size

0.1 mg

Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

The ARF tumour suppressor.

Gallagher SJ, Kefford RF, Rizos H
The international journal of biochemistry & cell biology 2006;38(10):1637-41

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Mule/ARF-BP1, a BH3-only E3 ubiquitin ligase, catalyzes the polyubiquitination of Mcl-1 and regulates apoptosis.

Zhong Q, Gao W, Du F, Wang X
Cell 2005 Jul 1;121(7):1085-95

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ARF-BP1/Mule is a critical mediator of the ARF tumor suppressor.

Chen D, Kon N, Li M, Zhang W, Qin J, Gu W
Cell 2005 Jul 1;121(7):1071-83

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 ?g/ml.

Immunocytochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunocytochemistry of ARF-BP1 in Daudi cells with AP30078PU-N ARF-BP1 antibody at 5 ?g/ml.

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Immunofluorescence of ARF-BP1 in Daudi cells with AP30078PU-N ARF-BP1 antibody at 20 ?g/ml.