Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-652 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-652, RRID:AB_2264587
- Product name
- Rabbit Polyclonal HUWE1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A Cancer-Specific Ubiquitin Ligase Drives mRNA Alternative Polyadenylation by Ubiquitinating the mRNA 3' End Processing Complex.
Prolyl hydroxylase 3 controls the intestine goblet cell generation through stabilizing ATOH1.
Infection with flaviviruses requires BCLXL for cell survival.
Human Antiviral Protein IFIX Suppresses Viral Gene Expression during Herpes Simplex Virus 1 (HSV-1) Infection and Is Counteracted by Virus-induced Proteasomal Degradation.
Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development.
HUWE1 interacts with PCNA to alleviate replication stress.
The double life of MULE in preeclamptic and IUGR placentae.
Aberrant expression of the apoptosis-related proteins BAK and MCL1 in T cells in multiple sclerosis.
Paracrine signaling through MYCN enhances tumor-vascular interactions in neuroblastoma.
Regulation of oxidative DNA damage repair by DNA polymerase λ and MutYH by cross-talk of phosphorylation and ubiquitination.
HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation.
Inactivation of arf-bp1 induces p53 activation and diabetic phenotypes in mice.
Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity.
Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival.
Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70.
Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.
Evidence for involvement of BH3-only proapoptotic members in adenovirus-induced apoptosis.
Yang SW, Li L, Connelly JP, Porter SN, Kodali K, Gan H, Park JM, Tacer KF, Tillman H, Peng J, Pruett-Miller SM, Li W, Potts PR
Molecular cell 2020 Mar 19;77(6):1206-1221.e7
Molecular cell 2020 Mar 19;77(6):1206-1221.e7
Prolyl hydroxylase 3 controls the intestine goblet cell generation through stabilizing ATOH1.
Xu YM, Gao Q, Zhang JZ, Lu YT, Xing DM, Qin YQ, Fang J
Cell death and differentiation 2020 Jul;27(7):2131-2142
Cell death and differentiation 2020 Jul;27(7):2131-2142
Infection with flaviviruses requires BCLXL for cell survival.
Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, Fukuhara T, Ikawa M, Satoh T, Yoshio S, Suzuki R, Saijo M, Huang DCS, Kanto T, Akira S, Matsuura Y
PLoS pathogens 2018 Sep;14(9):e1007299
PLoS pathogens 2018 Sep;14(9):e1007299
Human Antiviral Protein IFIX Suppresses Viral Gene Expression during Herpes Simplex Virus 1 (HSV-1) Infection and Is Counteracted by Virus-induced Proteasomal Degradation.
Crow MS, Cristea IM
Molecular & cellular proteomics : MCP 2017 Apr;16(4 suppl 1):S200-S214
Molecular & cellular proteomics : MCP 2017 Apr;16(4 suppl 1):S200-S214
Destabilization of Atoh1 by E3 Ubiquitin Ligase Huwe1 and Casein Kinase 1 Is Essential for Normal Sensory Hair Cell Development.
Cheng YF, Tong M, Edge AS
The Journal of biological chemistry 2016 Sep 30;291(40):21096-21109
The Journal of biological chemistry 2016 Sep 30;291(40):21096-21109
HUWE1 interacts with PCNA to alleviate replication stress.
Choe KN, Nicolae CM, Constantin D, Imamura Kawasawa Y, Delgado-Diaz MR, De S, Freire R, Smits VA, Moldovan GL
EMBO reports 2016 Jun;17(6):874-86
EMBO reports 2016 Jun;17(6):874-86
The double life of MULE in preeclamptic and IUGR placentae.
Rolfo A, Garcia J, Todros T, Post M, Caniggia I
Cell death & disease 2012 May 3;3:e305
Cell death & disease 2012 May 3;3:e305
Aberrant expression of the apoptosis-related proteins BAK and MCL1 in T cells in multiple sclerosis.
Mandel I, Paperna T, Miller A
Journal of neuroimmunology 2012 Mar;244(1-2):51-6
Journal of neuroimmunology 2012 Mar;244(1-2):51-6
Paracrine signaling through MYCN enhances tumor-vascular interactions in neuroblastoma.
Chanthery YH, Gustafson WC, Itsara M, Persson A, Hackett CS, Grimmer M, Charron E, Yakovenko S, Kim G, Matthay KK, Weiss WA
Science translational medicine 2012 Jan 4;4(115):115ra3
Science translational medicine 2012 Jan 4;4(115):115ra3
Regulation of oxidative DNA damage repair by DNA polymerase λ and MutYH by cross-talk of phosphorylation and ubiquitination.
Markkanen E, van Loon B, Ferrari E, Parsons JL, Dianov GL, Hübscher U
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 10;109(2):437-42
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 10;109(2):437-42
HUWE1 ubiquitinates MyoD and targets it for proteasomal degradation.
Noy T, Suad O, Taglicht D, Ciechanover A
Biochemical and biophysical research communications 2012 Feb 10;418(2):408-13
Biochemical and biophysical research communications 2012 Feb 10;418(2):408-13
Inactivation of arf-bp1 induces p53 activation and diabetic phenotypes in mice.
Kon N, Zhong J, Qiang L, Accili D, Gu W
The Journal of biological chemistry 2012 Feb 10;287(7):5102-11
The Journal of biological chemistry 2012 Feb 10;287(7):5102-11
Huwe1, a novel cellular interactor of Gag-Pol through integrase binding, negatively influences HIV-1 infectivity.
Yamamoto SP, Okawa K, Nakano T, Sano K, Ogawa K, Masuda T, Morikawa Y, Koyanagi Y, Suzuki Y
Microbes and infection 2011 Apr;13(4):339-49
Microbes and infection 2011 Apr;13(4):339-49
Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival.
Schwickart M, Huang X, Lill JR, Liu J, Ferrando R, French DM, Maecker H, O'Rourke K, Bazan F, Eastham-Anderson J, Yue P, Dornan D, Huang DC, Dixit VM
Nature 2010 Jan 7;463(7277):103-7
Nature 2010 Jan 7;463(7277):103-7
Regulation of heat-induced apoptosis by Mcl-1 degradation and its inhibition by Hsp70.
Stankiewicz AR, Livingstone AM, Mohseni N, Mosser DD
Cell death and differentiation 2009 Apr;16(4):638-47
Cell death and differentiation 2009 Apr;16(4):638-47
Cdc6 stability is regulated by the Huwe1 ubiquitin ligase after DNA damage.
Hall JR, Kow E, Nevis KR, Lu CK, Luce KS, Zhong Q, Cook JG
Molecular biology of the cell 2007 Sep;18(9):3340-50
Molecular biology of the cell 2007 Sep;18(9):3340-50
Evidence for involvement of BH3-only proapoptotic members in adenovirus-induced apoptosis.
Subramanian T, Vijayalingam S, Lomonosova E, Zhao LJ, Chinnadurai G
Journal of virology 2007 Oct;81(19):10486-95
Journal of virology 2007 Oct;81(19):10486-95
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP) from 293T cells. Ureb1/Lasu1 was less efficiently immunoprecipitated by another Ureb1/Lasu1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HUWE1 Antibody [NB100-652] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Lasu1/Ureb1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: HUWE1 Antibody [NB100-652] - Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Lasu1/Ureb1 antibody NB100-652 (lot NB100-652-9) used for IP at 6 ug per reaction. Lasu1/Ureb1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-652-8). For blotting immunoprecipitated Lasu1/Ureb1, NB100-652 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HUWE1 Antibody [NB100-652] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit antiLasu1/Ureb1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB.
