TA500715

antibody from OriGene

Targeting: ID3 bHLHb25, HEIR-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500715

Provider

OriGene

Proper citation

OriGene Cat#TA500715, RRID:AB_11126207

Product name

ID3 mouse monoclonal antibody, clone OTI4D1 (formerly 4D1)

Antibody type

Monoclonal

Description

ID3 mouse monoclonal antibody, clone OTI4D1 (formerly 4D1)

Host

Mouse

Conjugate

Unconjugated

Epitope

ID3

Isotype

IgG

Antibody clone number

OTI4D1

Vial size

100 µl

Concentration

0.56 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ID3 (RC200583, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ID3.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-ID3 mouse monoclonal antibody (TA500715) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ID3(RC200583).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-ID3 antibody(TA500715),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Hela cells, using anti-ID3 antibody(TA500715),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC