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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- H00003399-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003399-M01, RRID:AB_539738
- Product name
- Mouse Monoclonal ID3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ID3 - inhibitor of DNA binding 3, dominant negative helix-loop-helix protein
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ID3 Antibody (4G1) [H00003399-M01] - Analysis of ID3 over-expressed 293 cell line, cotransfected with ID3 Validated Chimera RNAi ( Cat # H00003399-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ID3 monoclonal antibody (M01), clone 4G1 (Cat # H00003399-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ID3 Antibody (4G1) [H00003399-M01] - ID3 monoclonal antibody (M01), clone 4G1 Analysis of ID3 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ID3 Antibody (4G1) [H00003399-M01] - Detection limit for recombinant GST tagged ID3 is approximately 0.03ng/ml as a capture antibody.
