Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 19424-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHCHD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.29 mg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- WB result of CHCHD2 antibody (19424-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HT-1080 cells were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 19424-1-AP ( CHCHD2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (Product # 19424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 19424-1-AP (CHCHD2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent analysis of HepG2 cells, using CHCHD2 antibody 19424-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19424-1-AP ( CHCHD2 Antibody) at dilution of 1:200 (under 10x lens).
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19424-1-AP ( CHCHD2 Antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- IP result of anti-CHCHD2 (IP:19424-1-AP, 4ug; Detection:19424-1-AP 1:1000) with HepG2 cells lysate 2520 ug.