MA5-24644

antibody from Invitrogen Antibodies

Targeting: MOG BTN6, BTNL11

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-24644

Provider

Invitrogen Antibodies

Product name

MOG Monoclonal Antibody (CL2852)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: ALVGDEVELP CRISPGKNAT GMEVGWYRPP FSRVVHLYRN GKDQDGDQAP EYRGRTELLK DAIGEGKVTL RIRNVRFSDE GGFTCFFRDH SYQEEAAMEL KVEDPFYWVS PG Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 89%. Binds to an epitope located within the peptide sequence FSRVVHLYRNGKDQD as determined by overlapping synthetic peptides.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

CL2852

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MOG in Lane1: Marker (kDa) 250, 130, 100, 70, 55, 35, 25, 15, 10, Lane 2: Human cerebral cortex tissuetissue. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-MOG Monoclonal Antibody (CL2852) (Product # MA5-24644) and a 28 kDa band corresponding to Myelin-oligodendrocyte glycoprotein was observed in Mouse, Rat Brain and Cerebellum but not in other tissues. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Cerebellum (Lane 4), Mouse Heart (Lane 5), Rat Heart (Lane 6), Mouse Liver (Lane 7), Rat Liver (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:2000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in rat cerebellum shows strong immunoreactivity in myelinated neural fibers. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in mouse cerebellum shows strong immunoreactivity in myelinated neural processes. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in human cerebral cortex tissue shows strong immunoreactivity in myelinated neural fibers. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in rat striatum shows strong positivity in myelinated neural processes. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in mouse corpus callosum shows strong immunoreactivity in myelinated neural fibers. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in human cerebellum shows strong immunoreactivity in myelinated neural processes. Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of MOG in human liver tissue shows absence of immunoreactivity (negative control). Samples were probed using a MOG Monoclonal Antibody (Product # MA5-24644).