PAB21207

antibody from Abnova Corporation

Targeting: PRCC RCCP1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21207

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21207, RRID:AB_10965360

Product name

PRCC polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant PRCC.

Antigen sequence

RNRGREEINFVEIKGDDQLSGAQQWMTKSLTEEKT
MKSFSKKKGEQPTGQQRRKHQITYLIHQAKERELE
LKNTWSENKLSRRQTQAKY


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with PRCC polyclonal antibody (Cat # PAB21207) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with PRCC polyclonal antibody (Cat # PAB21207) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum with PRCC polyclonal antibody (Cat # PAB21207) shows strong nuclear positivity in glandular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)