MA1-90154

antibody from Invitrogen Antibodies

Targeting: MYB c-myb

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-90154

Provider

Invitrogen Antibodies

Product name

Myb Monoclonal Antibody (IOA3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Predicted molecular weight of ~77 kDa.

Reactivity

Human, Mouse, Xenopus, Zebrafish

Host

Mouse

Isotype

IgG

Antibody clone number

IOA3

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Myb Monoclonal Antibody (IOA3) (Product # MA1-90154) and bands between 70-80 kDa corresponding to MYB was observed across the cell lines tested. The expression was decreased upon Trichostatin A treatment in Jurkat. Also, the expression was low in IM9 when compared to Jurkat. Modified whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), Jurkat treated with Trichostatin A (0.6uM for 16hrs) (Lane 3), K-562 (Lane 4), HeLa (Lane 5), IM9(Lane 6) and MDA-MB-231 (Lane 7) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).