A304-135A-T

antibody from Invitrogen Antibodies

Targeting: MYB c-myb

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A304-135A-T

Provider

Invitrogen Antibodies

Product name

c-Myb Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of mouse c-Myb by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- c-Myb antibody (Cat. No. A304-135A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-Myb by immunohistochemistry. Sample: FFPE section of human breast carcinoma (left) and salivary gland (right). Antibody: Affinity purified rabbit anti- c-Myb antibody (Cat. No. A304-135A Lot1) used at a dilution of 1:5,000 (0.2µg/ml) and 1:1,000 (1µg/ml). Detection: DAB.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human c-Myb by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from MOLT-4 cells. Antibodies: Affinity purified rabbit anti-c-Myb antibody A304-135A (lot A304-135A-1) used for IP at 6 µg/mg lysate. c-Myb was also immunoprecipitated by rabbit anti-c-Myb antibodies A304-134A, A304-136A and A304-137A. For blotting immunoprecipitated c-Myb, A304-138A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.