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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB10189 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10189, RRID:AB_1676366
- Product name
- Myb polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Myb.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Stress-induced inactivation of the c-Myb transcription factor through conjugation of SUMO-2/3 proteins.
Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity.
Sramko M, Markus J, Kabát J, Wolff L, Bies J
The Journal of biological chemistry 2006 Dec 29;281(52):40065-75
The Journal of biological chemistry 2006 Dec 29;281(52):40065-75
Covalent attachment of the SUMO-1 protein to the negative regulatory domain of the c-Myb transcription factor modifies its stability and transactivation capacity.
Bies J, Markus J, Wolff L
The Journal of biological chemistry 2002 Mar 15;277(11):8999-9009
The Journal of biological chemistry 2002 Mar 15;277(11):8999-9009
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Myb polyclonal antibody (Cat # PAB10189) in COS-7 cell lysates.Lane 1 : vector; Lane 2 : transfected with Myb, no treatment; Lane 3 : transfected with Myb and heat stress-treated (incubation at 42°C for 30 minutes) to induce Myb modification.Lane 3 shows detection of bands at 75, 98, and 125 KDa corresponding to overexpressed Myb (arrows) : wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.Nitrocellulose 0.45 um membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS.The primary antibody was used at 1:500.Personal communication, J. Bies, NCI NIH, Bethesda.
