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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09406PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09406PU-S, RRID:AB_2035362
- Product name
- anti MYB
- Antibody type
- Polyclonal
- Antigen
- Peptide corresponding to an internal portion of the c-Myb protein
- Reactivity
- Human, Mouse, Rat, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 25 µl
- Concentration
- 1.0 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-C-Myb antibody in Cos7 cell lysates: Lane 1: vector; Lane 2: transfected with c-myb, no treatment; Lane 3: transfected with c-Myb and heat stress-treated (incubation at 42οC for 30 minutes) to induce c-Myb modification. Lane 3 shows detection of bands at 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrows): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively. Nitrocellulose 0.45µm membrane was blocked for 1 hr at RT in 10% BLOTTO in PBS. The primary antibody was used at 1:500.
