Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF3140 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal MEPE/OF45 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human MEPE/OF45 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Cell Adhesion Mediated by MEPE and Neutralization by Human MEPE Antibody. Recombinant Human MEPE (Catalog # 3140-ME), immobil-ized onto a microplate, supports the adhesion of the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human MEPE (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human MEPE Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF3140). The ND50 is typically 2-6 µg/mL.