Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- HPA019849 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA019849, RRID:AB_1856000
- Product name
- Anti-RAB27B
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human RAB27B, Gene description: RAB27B, member RAS oncogene family, Validated applications: WB, IHC, Uniprot ID: O00194, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.2 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references RAB27B expression in pancreatic cancer is predictive of poor survival but good response to chemotherapy
RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells
A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies
Pecqueux M, Wende B, Sommer U, Baenke F, Oehme F, Hempel S, Aust D, Distler M, Weitz J, Kahlert C
Cancer Biomarkers 2023;37(4):207-215
Cancer Biomarkers 2023;37(4):207-215
RAB27B requirement for stretch-induced exocytosis in bladder umbrella cells
Gallo L, Dalghi M, Clayton D, Ruiz W, Khandelwal P, Apodaca G
American Journal of Physiology-Cell Physiology 2018;314(3):C349-C365
American Journal of Physiology-Cell Physiology 2018;314(3):C349-C365
A total transcriptome profiling method for plasma-derived extracellular vesicles: applications for liquid biopsies
Amorim M, Valieris R, Drummond R, Pizzi M, Freitas V, Sinigaglia-Coimbra R, Calin G, Pasqualini R, Arap W, Silva I, Dias-Neto E, Nunes D
Scientific Reports 2017;7(1)
Scientific Reports 2017;7(1)
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and RAB27B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418170).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human prostate and tonsil tissues using HPA019849 antibody. Corresponding RAB27B RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol