13412-1-AP

antibody from Proteintech Group

Targeting: RAB27B

Provider product page for 13412-1-AP

Western blot

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [39]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [2]

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Product number: 13412-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#13412-1-AP, RRID:AB_2176732
Product name: RAB27B antibody
Antibody type: Polyclonal
Description: KD/KO validated RAB27B antibody (Cat. #13412-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

RAB27B protein structure - 13412-1-AP shown in red.

Submitted references RAB31 in glioma-derived endothelial cells promotes glioma cell invasion via extracellular vesicle-mediated enrichment of MYO1C.

Suo J, Wang Y, Wang L, Qiu B, Wang Z, Yan A, Qiang B, Han W, Peng X
FEBS open bio 2024 Jan;14(1):138-147

Rab27a-mediated exosome secretion in anterior cingulate cortex contributes to colorectal visceral pain in adult mice with neonatal maternal deprivation.

Sun Q, Weng RX, Li JH, Li YC, Xu JT, Li R, Lu X, Xu GY
American journal of physiology. Gastrointestinal and liver physiology 2023 Oct 1;325(4):G356-G367

Multi-omic approach characterises the neuroprotective role of retromer in regulating lysosomal health.

Daly JL, Danson CM, Lewis PA, Zhao L, Riccardo S, Di Filippo L, Cacchiarelli D, Lee D, Cross SJ, Heesom KJ, Xiong WC, Ballabio A, Edgar JR, Cullen PJ
Nature communications 2023 May 29;14(1):3086

Pancreatic cancer-derived small extracellular vesical ezrin activates fibroblasts to exacerbate cancer metastasis through STAT3 and YAP-1 signaling pathways.

Chang YT, Peng HY, Hu CM, Tien SC, Chen YI, Jeng YM, Chang MC
Molecular oncology 2023 Aug;17(8):1628-1647

RAB27B Drives a Cancer Stem Cell Phenotype in NSCLC Cells Through Enhanced Extracellular Vesicle Secretion.

Meneses KM, Pandya P, Lindemann JA, Al-Qasrawi DS, Argo RA, Weems CM, Beetler DJ, Vijay GV, Yan IK, Wolfram J, Patel T, Justilien V
Cancer research communications 2023 Apr;3(4):607-620

Extracellular Vesicles Secreted by Mouse Decidual Cells Carry Critical Information for the Establishment of Pregnancy.

Ma Q, Beal JR, Song X, Bhurke A, Bagchi IC, Bagchi MK
Endocrinology 2022 Oct 23;163(12)

Whole-Transcriptome Sequence of Degenerative Meniscus Cells Unveiling Diagnostic Markers and Therapeutic Targets for Osteoarthritis.

Jiang Z, Du X, Wen X, Li H, Zeng A, Sun H, Hu S, He Q, Liao W, Zhang Z
Frontiers in genetics 2021;12:754421

NRF2 promotes urothelial cell response to bacterial infection by regulating reactive oxygen species and RAB27B expression.

Joshi CS, Mora A, Felder PA, Mysorekar IU
Cell reports 2021 Oct 19;37(3):109856

Silencing of long non-coding RNA SNHG15 suppresses proliferation, migration and invasion of pancreatic cancer cells by regulating the microRNA-345-5p/RAB27B axis.

Jiang P, Yin Y, Wu Y, Sun Z
Experimental and therapeutic medicine 2021 Nov;22(5):1273

hnRNPA2B1-Mediated Extracellular Vesicles Sorting of miR-122-5p Potentially Promotes Lung Cancer Progression.

Li C, Qin F, Wang W, Ni Y, Gao M, Guo M, Sun G
International journal of molecular sciences 2021 Nov 28;22(23)

Morphological and histochemical characterization of the secretory epithelium in the canine lacrimal gland.

Yasui T, Miyata K, Nakatsuka C, Tsukise A, Gomi H
European journal of histochemistry : EJH 2021 Nov 2;65(4)

LINC00511 drives invasive behavior in hepatocellular carcinoma by regulating exosome secretion and invadopodia formation.

Peng X, Li X, Yang S, Huang M, Wei S, Ma Y, Li Y, Wu B, Jin H, Li B, Tang S, Fan Q, Liu J, Yang L, Li H
Journal of experimental & clinical cancer research : CR 2021 Jun 4;40(1):183

FOXO1-Mediated Downregulation of RAB27B Leads to Decreased Exosome Secretion in Diabetic Kidneys.

Zeng M, Wen J, Ma Z, Xiao L, Liu Y, Kwon S, Liu Y, Dong Z
Diabetes 2021 Jul;70(7):1536-1548

IKKβ activation promotes amphisome formation and extracellular vesicle secretion in tumor cells.

Peng X, Yang L, Ma Y, Li X, Yang S, Li Y, Wu B, Tang S, Zhang F, Zhang B, Liu J, Li H
Biochimica et biophysica acta. Molecular cell research 2021 Jan;1868(1):118857

EHHADH contributes to cisplatin resistance through regulation by tumor-suppressive microRNAs in bladder cancer.

Okamura S, Yoshino H, Kuroshima K, Tsuruda M, Osako Y, Sakaguchi T, Yonemori M, Yamada Y, Tatarano S, Nakagawa M, Enokida H
BMC cancer 2021 Jan 11;21(1):48

CFAP43-mediated intra-manchette transport is required for sperm head shaping and flagella formation.

Yu Y, Wang J, Zhou L, Li H, Zheng B, Yang S
Zygote (Cambridge, England) 2021 Feb;29(1):75-81

Oncogenic effects of RAB27B through exosome independent function in renal cell carcinoma including sunitinib-resistant.

Tsuruda M, Yoshino H, Okamura S, Kuroshima K, Osako Y, Sakaguchi T, Sugita S, Tatarano S, Nakagawa M, Enokida H
PloS one 2020;15(5):e0232545

Androgen Receptor Signaling Reduces the Efficacy of Bacillus Calmette-Guérin Therapy for Bladder Cancer via Modulating Rab27b-Induced Exocytosis.

Mizushima T, Jiang G, Kawahara T, Li P, Han B, Inoue S, Ide H, Kato I, Jalalizadeh M, Miyagi E, Fukuda M, Reis LO, Miyamoto H
Molecular cancer therapeutics 2020 Sep;19(9):1930-1942

Mouse 4T1 Breast Cancer Cell-Derived Exosomes Induce Proinflammatory Cytokine Production in Macrophages via miR-183.

Guo J, Duan Z, Zhang C, Wang W, He H, Liu Y, Wu P, Wang S, Song M, Chen H, Chen C, Si Q, Xiang R, Luo Y
Journal of immunology (Baltimore, Md. : 1950) 2020 Nov 15;205(10):2916-2925

T cell-derived exosomes induced macrophage inflammatory protein-1α/β drive the trafficking of CD8(+) T cells in oral lichen planus.

Yang JY, Zhang J, Lu R, Tan YQ, Du GF, Zhou G
Journal of cellular and molecular medicine 2020 Dec;24(23):14086-14098

Inhibition of Rab27a and Rab27b Has Opposite Effects on the Regulation of Hair Cycle and Hair Growth.

Ku KE, Choi N, Sung JH
International journal of molecular sciences 2020 Aug 7;21(16)

Pannexin-1 limits the production of proinflammatory cytokines during necroptosis.

Douanne T, André-Grégoire G, Trillet K, Thys A, Papin A, Feyeux M, Hulin P, Chiron D, Gavard J, Bidère N
EMBO reports 2019 Oct 4;20(10):e47840

RAB27B-activated secretion of stem-like tumor exosomes delivers the biomarker microRNA-146a-5p, which promotes tumorigenesis and associates with an immunosuppressive tumor microenvironment in colorectal cancer.

Cheng WC, Liao TT, Lin CC, Yuan LE, Lan HY, Lin HH, Teng HW, Chang HC, Lin CH, Yang CY, Huang SC, Jiang JK, Yang SH, Yang MH, Hwang WL
International journal of cancer 2019 Oct 15;145(8):2209-2224

Exosome Release Is Regulated by mTORC1.

Zou W, Lai M, Zhang Y, Zheng L, Xing Z, Li T, Zou Z, Song Q, Zhao X, Xia L, Yang J, Liu A, Zhang H, Cui ZK, Jiang Y, Bai X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2019 Feb 6;6(3):1801313

Inactivation of Rab27B-dependent signaling pathway by calycosin inhibits migration and invasion of ER-negative breast cancer cells.

Wu G, Niu M, Qin J, Wang Y, Tian J
Gene 2019 Aug 15;709:48-55

Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.

Lu IL, Chen C, Tung CY, Chen HH, Pan JP, Chang CH, Cheng JS, Chen YA, Wang CH, Huang CW, Kang YN, Chang HY, Li LL, Chang KP, Shih YH, Lin CH, Kwan SY, Tsai JW
Nature communications 2018 Jun 27;9(1):2498

MiR-20a-5p promotes radio-resistance by targeting Rab27B in nasopharyngeal cancer cells.

Huang D, Bian G, Pan Y, Han X, Sun Y, Wang Y, Shen G, Cheng M, Fang X, Hu S
Cancer cell international 2017;17:32

Downregulation of serum RAB27B confers improved prognosis and is associated with hepatocellular carcinoma progression through PI3K-AKT-P21 signaling.

Yang X, Ye X, Sun L, Gao F, Li Y, Ji X, Wang X, Feng Y, Wang X
Oncotarget 2017 Sep 22;8(37):61118-61132

Canine Salivary Glands: Analysis of Rab and SNARE Protein Expression and SNARE Complex Formation With Diverse Tissue Properties.

Gomi H, Osawa H, Uno R, Yasui T, Hosaka M, Torii S, Tsukise A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2017 Nov;65(11):637-653

Exosomes maintain cellular homeostasis by excreting harmful DNA from cells.

Takahashi A, Okada R, Nagao K, Kawamata Y, Hanyu A, Yoshimoto S, Takasugi M, Watanabe S, Kanemaki MT, Obuse C, Hara E
Nature communications 2017 May 16;8:15287

Exophagy of annexin A2 via RAB11, RAB8A and RAB27A in IFN-γ-stimulated lung epithelial cells.

Chen YD, Fang YT, Cheng YL, Lin CF, Hsu LJ, Wang SY, Anderson R, Chang CP, Lin YS
Scientific reports 2017 Jul 18;7(1):5676

MLKL, the Protein that Mediates Necroptosis, Also Regulates Endosomal Trafficking and Extracellular Vesicle Generation.

Yoon S, Kovalenko A, Bogdanov K, Wallach D
Immunity 2017 Jul 18;47(1):51-65.e7

Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.

Chang YC, Su CY, Chen MH, Chen WS, Chen CL, Hsiao M
Molecular cancer 2017 Aug 7;16(1):135

Expression of ODC Antizyme Inhibitor 2 (AZIN2) in Human Secretory Cells and Tissues.

Rasila T, Lehtonen A, Kanerva K, Mäkitie LT, Haglund C, Andersson LC
PloS one 2016;11(3):e0151175

Hypomethylated Rab27b is a progression-associated prognostic biomarker of glioma regulating MMP-9 to promote invasion.

Wang H, Wang Y, Bao Z, Zhang C, Liu Y, Cai J, Jiang C
Oncology reports 2015 Sep;34(3):1503-9

Microenvironment-induced PTEN loss by exosomal microRNA primes brain metastasis outgrowth.

Zhang L, Zhang S, Yao J, Lowery FJ, Zhang Q, Huang WC, Li P, Li M, Wang X, Zhang C, Wang H, Ellis K, Cheerathodi M, McCarty JH, Palmieri D, Saunus J, Lakhani S, Huang S, Sahin AA, Aldape KD, Steeg PS, Yu D
Nature 2015 Nov 5;527(7576):100-104

Exosome transfer from stromal to breast cancer cells regulates therapy resistance pathways.

Boelens MC, Wu TJ, Nabet BY, Xu B, Qiu Y, Yoon T, Azzam DJ, Twyman-Saint Victor C, Wiemann BZ, Ishwaran H, Ter Brugge PJ, Jonkers J, Slingerland J, Minn AJ
Cell 2014 Oct 23;159(3):499-513

Unexpected gain of function for the scaffolding protein plectin due to mislocalization in pancreatic cancer.

Shin SJ, Smith JA, Rezniczek GA, Pan S, Chen R, Brentnall TA, Wiche G, Kelly KA
Proceedings of the National Academy of Sciences of the United States of America 2013 Nov 26;110(48):19414-9

Differential expression of Rab27A/B correlates with clinical outcome in hepatocellular carcinoma.

Dong WW, Mou Q, Chen J, Cui JT, Li WM, Xiao WH
World journal of gastroenterology 2012 Apr 21;18(15):1806-13

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: PC-3 cells were subjected to SDS PAGE followed by western blot with 13412-1-AP(RAB27B antibody) at dilution of 1:400
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human liver cancer using 13412-1-AP(RAB27B antibody) at dilution of 1:50 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The RAB27B antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human RAB27B. This antibody recognizes human,mouse,rat antigen. The RAB27B antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 13412-1-AP.pdf

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