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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10046 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10046, RRID:AB_1674782
- Product name
- FANCC polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of FANCC.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Functional relationships of FANCC to homologous recombination, translesion synthesis, and BLM.
The Fanconi anemia core complex associates with chromatin during S phase.
The Fanconi anemia proteins functionally interact with the protein kinase regulated by RNA (PKR).
Hirano S, Yamamoto K, Ishiai M, Yamazoe M, Seki M, Matsushita N, Ohzeki M, Yamashita YM, Arakawa H, Buerstedde JM, Enomoto T, Takeda S, Thompson LH, Takata M
The EMBO journal 2005 Jan 26;24(2):418-27
The EMBO journal 2005 Jan 26;24(2):418-27
The Fanconi anemia core complex associates with chromatin during S phase.
Mi J, Kupfer GM
Blood 2005 Jan 15;105(2):759-66
Blood 2005 Jan 15;105(2):759-66
The Fanconi anemia proteins functionally interact with the protein kinase regulated by RNA (PKR).
Zhang X, Li J, Sejas DP, Rathbun KR, Bagby GC, Pang Q
The Journal of biological chemistry 2004 Oct 15;279(42):43910-9
The Journal of biological chemistry 2004 Oct 15;279(42):43910-9
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using FANCC polyclonal antibody (Cat # PAB10046) shows detection of a band at ~63 KDa (arrowhead) corresponding to FANCC present in a HeLa whole cell lysate.The identity of the lower molecular weight band is unknown.Approximately 35 ug of lysate was separated by 4-20% Tris Glycine SDS-PAGE.After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to1:1,500 in PBS supplemented with 1% normal goatserum and 0.1% BLOTTO.The membrane was washed and reacted with a 1:10,000 dilution ofIRDye™800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature (800 nm channel, green).Molecular weight estimation was made bycomparison to prestained MW markers (indicated at left).IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
